Data is not available to indicate whether DNA contamination could affect sequencing. However, including DNase treatment is recommended to minimize genomic DNA in the sequencing reads.
STRN250
Spectrum™ Plant Total RNA Kit
sufficient for 250 purifications
동의어(들):
Plant total RNA extraction kit, Plant total RNA isolation kit, Plant total RNA purification kit
다음으로 건너뛰기
usage
sufficient for 250 purifications
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
RNA purification: suitable
Quality Level
test parameters
: ~30 min kit run time, sample volume: 100 mg
greener alternative category
storage temp.
15-25°C
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이 품목 | STRN50 | RTN350 | G2N70 |
|---|---|---|---|
| usage sufficient for 250 purifications | usage sufficient for 50 purifications | usage sufficient for 350 purifications | usage sufficient for 70 purifications |
| technique(s) RNA purification: suitable | technique(s) RNA purification: suitable | technique(s) RNA purification: suitable | technique(s) DNA purification: suitable |
| greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Inherently Safer Chemistry for Accident Prevention |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
STRN10 | STRN10, STRN250 | RTN10, RTN70, RTN9602, RTN9604 | G2N10, G2N350 |
| greener alternative category | greener alternative category | greener alternative category | greener alternative category , Aligned |
General description
The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.
After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.
Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.
Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry.
Application
Features and Benefits
- Yields up to 60 μg of pure concentrated RNA per prep
- Efficient protocol allows for RNA purification in 30 min or less
- Specially designed for research with difficult plant tissues
Legal Information
Spectrum is a trademark of Sigma-Aldrich Co. LLC
키트 구성품 전용
제품 번호
설명
- Binding Solution
- Lysis Solution
- Wash Solution 1
키트 구성품 역시 별도로 이용 가능함
제품 번호
설명
SDS
- M31482-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) 2
signalword
Danger
Hazard Classifications
Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2 Oral
target_organs
Liver,Heart
저장 등급
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
wgk
WGK 3
Zhonghua Li et al.
The New phytologist, 226(6), 1738-1752 (2020-02-06)
The cotton fibre serves as a valuable experimental system to study cell wall synthesis in plants, but our understanding of the genetic regulation of this process during fibre development remains limited. We performed a genome-wide association study (GWAS) and identified
Transcriptional stimulation of rate-limiting components of the autophagic pathway improves plant fitness
Minina EA, et al.
Journal of Experimental Botany, 69(6), 1415-1432 (2018)
Gregory S Jensen et al.
Journal of experimental botany, 68(3), 499-511 (2017-02-17)
Thigmomorphogenesis is a stereotypical developmental alteration in the plant body plan that can be induced by repeatedly touching plant organs. To unravel how plants sense and record multiple touch stimuli we performed a novel forward genetic screen based on the
Prasanta K Dash et al.
GM crops & food, 5(2), 106-119 (2014-07-30)
A robust phenotypic plasticity to ward off adverse environmental conditions determines performance and productivity in crop plants. Flax (linseed), is an important cash crop produced for natural textile fiber (linen) or oilseed with many health promoting products. This crop is
Anantha Peramuna et al.
Biochemical and biophysical research communications, 500(2), 418-422 (2018-04-17)
Securing a molecular toolbox including diverse promoters is essential for genome engineering. However, native promoters have limitations such as the available number or the length of the promoter. In this work, three short synthetic promoters were characterized by using the
관련 콘텐츠
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
세포, 조직, 혈액, 바이러스 및 기타 시료 유형으로부터 유전체 DNA, 플리스미드 DNA 및 총 RNA의 추출과 정제를 위한 일반적인 기법과 다운스트림 애플리케이션의 개요.
The Spectrum Plant Total RNA Kit incorporates a new, patent-pending lysis and binding chemistry. This kit is intended to purify total RNA from species and tissue where most Total RNA protocols fail. Without sacrificing affordability, you can purify more total RNA per isolation.
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I’m using your plant RNA extraction kit for RNA-seq. If I skip the DNase treatment, could DNA contamination affect the sequencing library, or is it mandatory to include this step? Thank you.
1 답변-
도움이 되었습니까?
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does it sufficient for small RNA and/or non-coding RNA extraction?
1 답변-
Yes, this kit is sufficient for small RNA. However, It is worth noting that small RNA molecules, such as microRNA, siRNA, tRNA, 5S rRNA, are not efficiently recovered using the standard procedure. If desired, these small RNA molecules can be recovered by increasing the amount of binding solution, as described in Protocol A under Procedure Step 4.
For more information, please see the protocol associated with this product:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/323/177/strn250bul.pdf도움이 되었습니까?
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