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Merck
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주요 문서

Z380059

Sigma-Aldrich

Breathe-Easy® sealing membrane

Breathe-Easy® sealing membrane
2명의 제품 평가자(총 6명)가 샘플 제품을 받았거나 프로모션에 참여했습니다

polyurethane membrane with acrylic adhesive pre-cut to fit standard multiwell plates.

동의어(들):

breathable sealing membrane, cell culture, microplates, microwell plates, multi-well plates, multi-well sealing film, plate sealers, plate sealing film, sealing film, tissue culture

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크기 선택

100 EA
₩278,719

₩278,719


예상 입고일2025년 2월 10일세부사항



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보기 변경
100 EA
₩278,719

About This Item

UNSPSC 코드:
41122410
NACRES:
NB.22

₩278,719


예상 입고일2025년 2월 10일세부사항


material

rectangular transparent membrane (polyurethane)

무균

sterile

특징

piercable

포장

pack of 100 ea

제조업체/상표

Diversified Biotech BEM-1

일반 설명

Breathe-Easy® membrane is permeable to oxygen, carbon dioxide and water vapor, creating separate, highly uniform microculture vessels from a multi-well plate.
The membrane comes as a sandwich. The bottom paper liner protects the adhesive layer, and the top carrier sheet protects the outer surface of the highly compliant membrane until it has been placed and adhered to the plate. The liners also assure sterility of each individual membrane. The adhesive seals every well (unlike molded plastic covers), permitting agitation without cross-contamination or microbial infiltration. Compatible with most caustics and organic solvents, stable from −80 to +100 °C.
Note: Use of the Brayer (Z380067 sold separately) is recommended to assure uniform adhesion and to facilitate removal of the top paper liner.

특징 및 장점

Breathe-Easy® memebrane:
  • Sterile & Non-Cytotoxic: Sterilized by gamma irradiation (free of cytotoxins)
  • Prevent Contamination: Films eliminate well-to-well contamination, microbial and viral contamination, evaporation (excluding breathable membranes) and sample spillage
  • Ideally Sized and Designed: 6" x 3.25" rectangles fit all microplates. Two scored end-tabs make film positioning and removal hassle-free
  • Thermal Stability: Designed to withstand a wide temperature range
  • Easy Specimen Retrieval: Pierceable sealing films facilitate easy removal of samples with a pipet tip

법적 정보

Breathe-Easy is a registered trademark of Diversified Biotech

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시험 성적서(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 문서 section.

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이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Victoria Hsiao et al.
ACS synthetic biology, 4(2), 150-161 (2014-05-23)
As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this
Adithi R Varadarajan et al.
NPJ biofilms and microbiomes, 6(1), 46-46 (2020-11-01)
Pseudomonas aeruginosa MPAO1 is the parental strain of the widely utilized transposon mutant collection for this important clinical pathogen. Here, we validate a model system to identify genes involved in biofilm growth and biofilm-associated antibiotic resistance. Our model employs a
Teng Wang et al.
Nature communications, 11(1), 5589-5589 (2020-11-06)
Conjugative plasmids can mediate the spread and maintenance of diverse traits and functions in microbial communities. This role depends on the plasmid's ability to persist in a population. However, for a community consisting of multiple populations transferring multiple plasmids, the
Nathan A Sallee et al.
SLAS discovery : advancing life sciences R & D, 25(9), 1047-1063 (2020-07-28)
The identification of novel peptide hormones by functional screening is challenging because posttranslational processing is frequently required to generate biologically active hormones from inactive precursors. We developed an approach for functional screening of novel potential hormones by expressing them in
Filipe Pinto et al.
Nature communications, 11(1), 1529-1529 (2020-04-07)
Inteins are protein segments capable of joining adjacent residues via a peptide bond. In this process known as protein splicing, the intein itself is not present in the final sequence, thus achieving scarless peptide ligation. Here, we assess the splicing

질문

1–4 / 4 질문  
  1. Is this product optically transparent? Can it be used for luminescence experiments?

    1 답변
    1. This product is distributed on behalf of Diversified Biotech. The films have not been tested for use in luminescence, but are UV transparent to 300 nm.

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  2. Ces films sont-ils adaptés pour de la culture cellulaire? Remplacent-ils le couvercle ou doit-on mettre un film (stérile?) et le couvercle de la plaque dessus?

    1 답변
    1. Yes, these sealing films are sterile and suitable for use in cell culture. When in place, a plate lid is not needed. These films allow for the exchange of oxygen, carbon dioxide, and water vapors while preventing microbial/viral contamination and well-to-well cross contamination.

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  3. Is the Breath-easy sealing meant to be used with or without a lid? does it prevent contamination from leaking in without a lid?

    1 답변
    1. The adhesive seals are meant to be used without a lid. These items provide a secure seal for every well which prevents microbial/viral contamination and well-to-well cross-contamination.

      도움이 되었습니까?

  4. Is it plate reader friendly for absorbance wavelength

    1 답변
    1. This product is distributed on behalf of Diversified Biotech. The original manufacturer states that these films are UV transparent to 300 nm.

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후기

2명의 제품 평가자(총 6명)가 샘플 제품을 받았거나 프로모션에 참여했습니다
1–3 / 6 상품평  

활성 필터

    • 상품평 1
    • 투표 9
    별 5개 중 1개입니다.

    transparent breath easy sealing membranes for microtiter readers

    We bought the Z380059, Breathe-Easy® sealing membrane to measure the growth curve of aerobic bacteria in microtiter plate. From the description of the product it sounds like this is the application they are sold for and in the picture from the producer the membranes look totally transparent. But when we received the product the membranes were rather opaque and could not be used for cell density reading. We contacted the supplier and received this answer: "The manufacturer of Z380059, Breathe-Easy® sealing membrane, has advised that these films are not transparent. Unfortunately we do not have any sealing films that are both breathable and optically clear."

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    도움이 되었습니까?

    1. Sigma-Aldrich Corporation의 답변:

      Thank you for your review. We are sorry to hear that your experience did not match your product expectations. We encourage customers who experience problems with our products to call or email us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

  1. SF
    • 상품평 1
    • 투표 2
    별 5개 중 5개입니다.

    superior performance at -80C

    i love this product for its reliable performance, especially at -80 degree C

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    • 상품평 1
    • 투표 0
    별 5개 중 1개입니다.

    A lot of air bubbles and media leaking out on 384 well plates

    We used the Breath-Easy with a plate lid because we were afraid of contamination. When we came on the next day it was a big mess of media leaking out, air bubbles and definitely cross contamination between the wells. Is the Breath-easy sealing meant to be used without a lid? without contamination risk?

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    도움이 되었습니까?

    1. Sigma-Aldrich Corporation의 답변:

      Breathe-Easy® sealing membranes are intended to prevent cross contamination and do not require use of a lid. When applying the seal, take care to press the adhesive to the cell openings to make sure there is a tight seal on each well.

      We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

  2. 3 평점-상품평만

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