Protein quantitation

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facet applications:Protein quantitation

facet content type:Protocol

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Guide to create custom PLA probes for immunofluorescent or brightfield detection.

Duolink® PLA Multicolor Detection Protocol

Using Duolink® in Protein Interaction Studies

The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.

LC-UV-MS Method Development for Antibody Drug Conjugates Using a Non-Toxic ADC-Mimic

SigmaMab Antibody Drug Conjugate Mimic, is a non-toxic drug mimic utilized as a standard for mass spectrometry and high performance liquid chromatography.

Counterstaining after the Duolink In Situ Protocol

We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.

Duolink® In Situ Short Instructions - Fluorescence

This page details the Duolink® In Situ Short Protocol for fluorescence detection

Protein Determination by the Bicinchoninic Acid (BCA) Method

A standard procedure for the quantitative determination of total protein concentration of a solution by the BCA method.

Protein Determination using 2-D Quant Kit

2-D Quant Kit from Cytiva is designed to accurately determine protein concentrations in samples for electrophoresis.

Protein Determination Modified Lowry Method

To standardize a procedure for the determination of protein by modified Lowry.

Production of Dual-labelled Proteins

Refinement of protein dual-labeling protocol using EnPresso® B Defined Nitrogen-free showcased by Vernalis, Ltd. for drug discovery.

Creating PLA® Probes

Duolink® kits use in situ PLA® technology for accurate quantification of individual proteins, interactions, and modifications in cells and tissue.

Duolink® PLA Brightfield Protocol

Duolink® PLA reagents enable brightfield detection and quantification of proteins and interactions in tissue samples.

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.