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SPE에서는 액체 시료 내 하나 이상의 분석물이 고체 고정상에 추출, 분배 및/또는 흡착되어 분리된다. * * * ## 관련 제품 Slide 1 of 22 1 of 6 [ \ Supelco \ 575659-U \ HybridSPE®-PLus 96-Well Plate](https://www.sigmaaldrich.com/KR/ko/product/supelco/575659u) 빠른 보기 [ \ Supelco \ 57057 \ Supelclean™ LC-Florisil® SPE Tube](https://www.sigmaaldrich.com/KR/ko/product/supelco/57057) 빠른 보기 [ \ Supelco \ 57226 \ Supelclean™ENVI™-Chrom P SPE Tube](https://www.sigmaaldrich.com/KR/ko/product/supelco/57226) 빠른 보기 [ \ Supelco \ 54024-U \ Supelclean™ ENVI-Carb/NH2 SPE Tube](https://www.sigmaaldrich.com/KR/ko/product/supelco/54024u) 빠른 보기 [ \ Supelco \ 54035-U \ Supelclean™ ENVI-Carb/NH2 SPE Tube](https://www.sigmaaldrich.com/KR/ko/product/supelco/54035u) 빠른 보기 [ \ Supelco \ 57088 \ Supelclean™ ENVI-Carb™ SPE Tube](https://www.sigmaaldrich.com/KR/ko/product/supelco/57088) 빠른 보기 [ \ Supelco \ 57094 \ Supelclean™ ENVI-Carb™ SPE Tube](https://www.sigmaaldrich.com/KR/ko/product/supelco/57094) 빠른 보기 [ \ Supelco \ 52738-U \ Supelclean™ PSA SPE Bulk Packing](https://www.sigmaaldrich.com/KR/ko/product/supelco/52738u) 빠른 보기 [ \ Supelco \ 57210-U \ Supelclean™ ENVI-Carb™ SPE Bulk Packing](https://www.sigmaaldrich.com/KR/ko/product/supelco/57210u) 빠른 보기 [ \ Supelco \ 28399-U \ Dual-Layer Carbon Reversible Tube (Micro-Column)](https://www.sigmaaldrich.com/KR/ko/product/supelco/28399u) 빠른 보기 [ \ Supelco \ 28397-U \ Multi-Layer Silica Gel Dioxin Column](https://www.sigmaaldrich.com/KR/ko/product/supelco/28397u) 빠른 보기 [ \ Supelco \ 57044 \ Visiprep™ SPE Vacuum Manifold](https://www.sigmaaldrich.com/KR/ko/product/supelco/57044) 빠른 보기 [ \ Supelco \ 57265 \ Visiprep™ SPE Vacuum Manifold](https://www.sigmaaldrich.com/KR/ko/product/supelco/57265) 빠른 보기 [ \ Supelco \ 57493-U \ Supel™ Swift HLB SPE Tubes](https://www.sigmaaldrich.com/KR/ko/product/supelco/57493u) 빠른 보기 [ \ Supelco \ 55274-U \ Supel™Genie C8 Online SPE Starter Kit](https://www.sigmaaldrich.com/KR/ko/product/supelco/55274u) 빠른 보기 [ \ Supelco \ 55228-U \ Supel™ QuE](https://www.sigmaaldrich.com/KR/ko/product/supelco/55228u) 빠른 보기 [ \ Supelco \ 55227-U \ Supel™ QuE](https://www.sigmaaldrich.com/KR/ko/product/supelco/55227u) 빠른 보기 [ \ Supelco \ 52977-U \ HybridSPE® DPX Tip](https://www.sigmaaldrich.com/KR/ko/product/supelco/52977u) 빠른 보기 [ \ Supelco \ 575608-U \ Discovery® DSC-Si SPE 96-well Plate](https://www.sigmaaldrich.com/KR/ko/product/supelco/575608u) 빠른 보기 [ \ Supelco \ 54341-U \ Supelclean™ EZ-POP NP](https://www.sigmaaldrich.com/KR/ko/product/supelco/54341u) 빠른 보기 [ \ Supelco \ 55324-U \ Supel™Genie HybridSPE® Online SPE Starter Kit](https://www.sigmaaldrich.com/KR/ko/product/supelco/55324u) 빠른 보기 [ \ Supelco \ 52974-U \ HybridSPE® DPX Tip](https://www.sigmaaldrich.com/KR/ko/product/supelco/52974u) 빠른 보기 * * * ## 추천 카테고리 [](https://www.sigmaaldrich.com/KR/ko/products/analytical-chemistry/analytical-sample-preparation/spe-and-quechers) [고상 추출(SPE) 및 QuEChERS](https://www.sigmaaldrich.com/KR/ko/products/analytical-chemistry/analytical-sample-preparation/spe-and-quechers) 고체상 추출(SPE) 및 QuEChERS 시료 전처리를 위한 다양한 재료를 만나보세요. 카트리지, 튜브, 플레이트 등 다양한 제품이 준비되어 있습니다. [제품 쇼핑하기](https://www.sigmaaldrich.com/KR/ko/products/analytical-chemistry/analytical-sample-preparation/spe-and-quechers) [](https://www.sigmaaldrich.com/KR/ko/products/analytical-chemistry/analytical-chromatography/hplc-columns) [HPLC 컬럼](https://www.sigmaaldrich.com/KR/ko/products/analytical-chemistry/analytical-chromatography/hplc-columns) 당사의 광범위한 HPLC 컬럼 컬렉션으로 정밀한 분리를 달성하십시오. 유지력, 분해능 및 선택성을 향상시키십시오. 지금 주문하십시오.   [제품 쇼핑하기](https://www.sigmaaldrich.com/KR/ko/products/analytical-chemistry/analytical-chromatography/hplc-columns) [](https://www.sigmaaldrich.com/KR/ko/products/analytical-chemistry/analytical-chromatography/gc-columns-accessories-and-adsorbents) [GC 컬럼, 액세서리 및 흡착제](https://www.sigmaaldrich.com/KR/ko/products/analytical-chemistry/analytical-chromatography/gc-columns-accessories-and-adsorbents) 당사의 컬럼, 소모품 및 액세서리로 우수한 GC 성능을 달성하십시오. Supelco® 제품군을 살펴보십시오. [제품 쇼핑하기](https://www.sigmaaldrich.com/KR/ko/products/analytical-chemistry/analytical-chromatography/gc-columns-accessories-and-adsorbents) * * * 개요 관련 기사 및 프로토콜 지원 고상 추출(SPE)은 크로마토그래피 분석(예: HPLC, GC, TLC) 전 신속하고 선택적인 시료 전처리 및 정제를 위해 고안된 기술이다. SPE에서는 액체 시료 내 하나 이상의 분석물이 고체 고정상에 추출, 분배 및/또는 흡착되어 분리된다. SPE를 통한 시료 전처리는 시료의 원래 매트릭스를 더 단순한 매트릭스 환경으로 변화시킵니다. 이는 시료를 후속 분석 크로마토그래피에 더 적합하게 만들어 최종 정성 및 정량 분석을 단순화하고 개선하는 경우가 많습니다. 단순화된 시료 매트릭스는 분석 시스템에 가해지는 부담을 줄여 시스템 수명을 연장할 가능성도 있습니다. 최적의 SPE 절차는 다음과 같은 이점을 제공합니다: - 표적 크로마토그래피 방법과 더 호환되도록 시료 매트릭스를 전환합니다. - 분석물 농축(미량 농축)을 통한 감도 향상. - 크로마토그래피 분석 중 높은 배경, 오해의 소지가 있는 피크 및/또는 낮은 감도를 유발하는 간섭물질을 제거합니다. - 오염물질로부터 분석 컬럼을 보호합니다. - 추출 과정을 자동화합니다. ## SPE는 어떻게 작동하나요? SPE에서는 고정상(흡착제 또는 수지)이 분석 대상 물질 또는 불순물을 강력하지만 가역적인 상호작용을 통해 결합하여 복잡한 시료에서 관심 분석 물질을 안정적이고 신속하게 추출합니다. 다양한 분석물과 매트릭스에 대해 다양한 흡착제와 용출 조건을 사용할 수 있으므로 SPE는 선택적이고 다용도로 사용됩니다. 일반적인 SPE 흡착제는 다음과 같습니다: - 실리카 기반 - 역상(C18, C8, 시아노, 페닐) - 정상상 (실리카, 디올,NH2) - 이온 교환 (SAX, WCX, SCX) - 탄소 기반 - 폴리머 기반 (다양한 조성, 다양한 기능성) - 기타, 예를 들어플로리실® (마그네슘 실리케이트) 또는 알루미나 - 혼합층: 위의 거의 모든 것들을 순차적 층으로 조합한 것 ## SPE 전략 '결합-탈착 SPE'에서는 관심 분석물이 흡착제에 포집되고 매트릭스 간섭 물질은 카트리지를 통과합니다. '간섭 제거 SPE'에서는 매트릭스 간섭 물질이 흡착제에 포집되고 관심 분석물이 통과합니다. 하이브리드SPE(HybridSPE)와[퀀티파이드](https://www.sigmaaldrich.com/KR/ko/applications/analytical-chemistry/sample-preparation/quechers) 추출[(](https://www.sigmaaldrich.com/KR/ko/applications/analytical-chemistry/sample-preparation/quechers)[](https://www.sigmaaldrich.com)[) QuEChERS](https://www.sigmaaldrich.com/KR/ko/applications/analytical-chemistry/sample-preparation/quechers) SPE 방법은 모두 간섭 제거 방식을 통해 작동합니다. 최적의 SPE 방법은 분석물의 구조, 용해도, 극성 및 친유성 특성(분배 계수)에 따라 달라집니다. 관심 있는 응용 분야에 가장 적합한 고정상 및 용매를 선택하는 데 도움이 되는 [](https://www.sigmaaldrich.com)선택 가이드를 이용할 수 있습니다. ## SPE의 일반적인 적용 분야 SPE는 제약, 임상 및 고처리량 진단 검사, 법의학, 환경, 식품/농화학 산업에서 다음과 관련된 분석에 자주 사용됩니다: - 생물학적 유체 내 의약품 화합물 및 대사산물 - 생체액 내 약물 남용 물질 - 음용수 및 폐수 내 환경 오염물질 - 식품/농업 매트릭스 내 농약, 항생제 또는 미코톡신 - 단백질 및 펩타이드의 탈염 - 지질 분획 - 수용성 및 지용성 비타민 [](https://www.sigmaaldrich.com/documents-search) [더 구체적인 정보를 찾고 계신가요?](https://www.sigmaaldrich.com/documents-search) 데이터 시트, 인증서 및 기술 문서를 확인하려면 문서 검색 페이지를 방문하십시오. [문서 찾기](https://www.sigmaaldrich.com/documents-search) ## 관련 기사 - [생물학적 SPME를 이용한 시료 청정도 분석](https://www.sigmaaldrich.com/KR/ko/technical-documents/technical-article/analytical-chemistry/solid-phase-microextraction/sample-cleanliness-using-biospme) 인간 혈장에서 LC-MS/MS 분석을 위한 최소한의 매트릭스 효과를 가진 깨끗한 시료를 준비하기 위한 Supel™ BioSPME C18 96-핀 도구에 대해 알아보고, 이를 아세토니트릴 기반 침전법과 비교합니다. - [미국 환경보호청(EPA) 1633에 따른 수중 PFAS 분석](https://www.sigmaaldrich.com/KR/ko/technical-documents/technical-article/environmental-testing-and-industrial-hygiene/waste-water-and-process-water-testing/analysis-of-pfas-in-water-according-to-epa-1633) 미국 환경보호청(EPA) 방법 1633에 따라 Supelclean™ ENVI-WAX™ SPE 카트리지를 사용하여 수질 시료 내 퍼플루오로알킬 및 폴리플루오로알킬 물질(PFAS)의 LC-MS/MS 분석. - [Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC](https://www.sigmaaldrich.com/KR/en/technical-documents/protocol/environmental-testing-and-industrial-hygiene/air-testing/aldehydes-air-dnph) Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection. - [Effect of Container Materials on Milk Off-Flavors](https://www.sigmaaldrich.com/KR/en/technical-documents/technical-article/analytical-chemistry/gas-chromatography/container-materials) HS-SPME with Carboxen®/PDMS coated Nitinol fiber allows low MW analyte detection in light-exposed milk plastics. - [Pesticide Residue Analysis in Ginger Powder with QuEChERS](https://www.sigmaaldrich.com/KR/en/technical-documents/technical-article/analytical-chemistry/quechers/analysis-of-pesticide-residues-in-ginger-powder) 2-layer SPE cartridge efficiently reduces background for ginger contaminant analysis. - [모두 보기 (43)](https://www.sigmaaldrich.com/KR/ko/search/facet-search?focus=sitecontent&term=facet-search) ## 관련 프로토콜 - [음용수 내 Bisphenol A 측정](https://www.sigmaaldrich.com/KR/ko/technical-documents/protocol/environmental-testing-and-industrial-hygiene/drinking-water-testing/bisphenol-a-water) Supelco는 음용수 내 BPA 측정을 위한 종합적인 시료 조제 및 분석 절차를 개발했습니다. - [환경 용수 내 Triclosan 측정](https://www.sigmaaldrich.com/KR/ko/technical-documents/protocol/environmental-testing-and-industrial-hygiene/waste-water-and-process-water-testing/determination-triclosan-water) Triclosan은 HPLC 후에 UV 및 MS/MS 검출을 이용하여 폐수처리시설의 배출수에서 검출되었습니다. - [Empowering Biochemists to Analyze Glycoproteins with Exceptional Reproducibility](https://www.sigmaaldrich.com/KR/en/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/bioshell-glycan-hplc-columns) Fast, high-resolution separations of procainamide labeled glycans - dextran ladder, human IgG and cetuximab - [Biological Testing for Amphetamine and Methamphetamine Abuse](https://www.sigmaaldrich.com/KR/en/technical-documents/protocol/analytical-chemistry/small-molecule-hplc/biological-testing-for-amphetamine-and-methamphetamine-abuse) In this study, optimized methods are presented for sample preparation and chiral chromatography for the LC/MS analysis of amphetamine and methamphetamine enantiomers in urine. - [Accurate Biological Testing for Amphetamine and Methamphetamine Abuse Using Chiral HPLC and MS Detection](https://www.sigmaaldrich.com/KR/en/technical-documents/protocol/analytical-chemistry/small-molecule-hplc/chiral-hplc-amphetamine-methamphetamine) Optimized sample prep and chiral chromatography methods for the LC/MS analysis of these drug enantiomers in urine - [모두 보기 (34)](https://www.sigmaaldrich.com/KR/ko/search/facet-search?focus=sitecontent&term=facet-search) ### 더 많은 기사와 프로토콜 찾기 키워드 입력 검색 ## 어떻게 도와드릴까요? 문의 사항이 있으시면 [고객 지원 요청을](https://www.sigmaaldrich.com/KR/ko/support/customer-support) 제출해 주시기 바랍니다 또는 고객 서비스 팀에 문의해 주세요: 이메일: [[email protected]](mailto:[email protected]) 또는 전화: +1 (800) 244-1173 ## 추가 지원 - [Chromatogram Search](https://www.sigmaaldrich.com/chromatogram-search) Use the Chromatogram Search to identify unknown compounds in your sample. - [계산기 및 앱](https://www.sigmaaldrich.com/KR/ko/support/calculators-and-apps) 웹 툴박스 - 분석 화학, 생명과학, 화학 합성 및 재료 과학을 위한 과학 연구 도구 및 리소스. - [Customer Support Request](https://www.sigmaaldrich.com/KR/ko/support/customer-support) 씨그마 알드리치의 주문, 제품, 계정 및 웹사이트에 대해 궁금하신 부분을 확인할 수 있습니다 - [FAQ](https://maestro.my.site.com/knowledgeportal/s/) Explore our Frequently Asked Questions for answers to commonly asked questions about our products and services. * * * __추천 기사__ [Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC](https://www.sigmaaldrich.com/KR/en/technical-documents/protocol/environmental-testing-and-industrial-hygiene/air-testing/aldehydes-air-dnph) Carbonyl compounds react within the DNPH cartridge to form immobilized hydrazones, which can be subjected to automated desorption and HPLC analysis with UV detection. [Effect of Container Materials on Milk Off-Flavors](https://www.sigmaaldrich.com/KR/en/technical-documents/technical-article/analytical-chemistry/gas-chromatography/container-materials) HS-SPME with Carboxen®/PDMS coated Nitinol fiber allows low MW analyte detection in light-exposed milk plastics. [Pesticide Residue Analysis in Ginger Powder with QuEChERS](https://www.sigmaaldrich.com/KR/en/technical-documents/technical-article/analytical-chemistry/quechers/analysis-of-pesticide-residues-in-ginger-powder) 2-layer SPE cartridge efficiently reduces background for ginger contaminant analysis. 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