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Bouin′s solution

Name: Bouin′s solution
Brand: SIGMA

histological fixative

Synonym(s):

Bouin’s fluid

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About This Item

UNSPSC Code:

12171500

NACRES:

NA.47

MDL number:

MFCD00146169

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Properties

Quality Segment

200

shelf life

Expiry date on the label

composition

acetic acid, 5% , formaldehyde, 9% , picric acid, 0.9%

color

yellow

application(s)

hematology
histology

storage temp.

room temp

Description

General description

Bouin′s solution is a noncoagulant yellow stain fixative. It imposes shrinkage on tissues when used as preservative due to its picric acid component. It is majorly used in ureteroscopic biopsies. It preserves morphological and nuclear information in tissues upon fixation.

Application

Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures.

Bouin′s solution has been used:

in the staining of human liver biopsy tissue samples for two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging
in the fixation of rat ovaries for the observing follicle cells using microscopy
in the fixation of gonads of crab for morphological and histological analysis

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This Item	32857	21819	HT501320
Sigma-Aldrich HT101128 Bouin′s solution	Sigma-Aldrich 32857 Wright Stain solution	Sigma-Aldrich 21819 Carbol-Fuchsin solution according to Kinyoun	Sigma-Aldrich HT501320 Formalin solution, neutral buffered, 10%
description histological fixative	description according to Wright	description suitable for microscopy	description histological tissue fixative
color yellow	color -	color red to very dark red	color colorless
application(s) hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) diagnostic assay manufacturing hematology histology	application(s) hematology histology
composition formaldehyde, 9% , acetic acid, 5% , picric acid, 0.9%	composition -	composition fuchsin basic (certified stain; C.I. 42500), 3 % (w/w)	composition -
shelf life Expiry date on the label	shelf life -	shelf life -	shelf life -
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level -
storage temp. room temp	storage temp. room temp	storage temp. room temp	storage temp. room temp

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