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Merck

Application of the Fenton process in the elimination of helminth eggs.

Journal of water and health (2014-12-05)
Sandra Isabel Escobar-Megchún, Hugo Alejandro Nájera-Aguilar, Magaly González-Hilerio, Javier Gutiérrez-Jiménez, Rubén Fernando Gutiérrez-Hernández, María Neftalí Rojas-Valencia
PMID25473981
ABSTRACT

This study relates to a method for evaluating the degradation efficiency of Ascaris lumbricoides eggs through a Fenton reaction, using hydrogen peroxide (H2O2) at 100, 250, and 500 mg/L, ferrous sulfate (FeSO4) at 458 mg/L Fe(2+) and pH values of 3, 4.5, and 6. The experiments were conducted according to a 3(2) experimental design, with 1:1, 3:1, and 5:1 (H2O2/Fe(2+)) molar ratios. The oxidation and flocculation stages were performed at 130 rpm during 2 hours and at 25-30 rpm during 20 min, respectively. As a result of the Fenton reaction, an average of 91.2% Ascaris lumbricoides egg degradation was achieved at pH 6 using a 500 mg/L dose of H2O2 and a 3:1 (H2O2/Fe(2+)) molar ratio. Thus, this process is an alternative for eliminating parasites that are resistant to conventional disinfection processes and significantly reduces the human health hazard they represent.

MATERIALS
Product Number
Brand
Product Description
16911
Supelco
Hydrogen peroxide solution, 30 % (w/w), for ultratrace analysis
209309
Sigma-Aldrich
Iron, powder, −325 mesh, 97%
356808
Sigma-Aldrich
Iron, foil, thickness 0.1 mm, ≥99.9% trace metals basis
267945
Sigma-Aldrich
Iron, chips, 99.98% trace metals basis
44890
Sigma-Aldrich
Iron, puriss. p.a., carbonyl-Iron powder, low in magnesium and manganese compounds, ≥99.5% (RT)
88597
Millipore
Hydrogen peroxide solution, 3%, suitable for microbiology
IRMM524B
Iron, IRMM®, certified reference material, 0.5 mm wire
95321
Supelco
Hydrogen peroxide solution, ≥30%, for trace analysis
18304
Sigma-Aldrich
Hydrogen peroxide solution, 34.5-36.5%
95294
Sigma-Aldrich
Hydrogen peroxide solution, tested according to Ph. Eur.
F8263
Sigma-Aldrich
Iron(II) sulfate heptahydrate, suitable for plant cell culture, ≥99%
F8633
Sigma-Aldrich
Iron(II) sulfate heptahydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%
H1009
Sigma-Aldrich
Hydrogen peroxide solution, 30 % (w/w) in H2O, contains stabilizer
GF46206514
Iron, microfoil, disks, 10mm, thinness 0.05μm, specific density 40.44μg/cm2, permanent mylar 3.5μm support, 99.99+%
GF30656077
Iron, tube, 200mm, outside diameter 10.0mm, inside diameter 7mm, wall thickness 1.5mm, annealed, 99.5%
GF42997696
Iron, foil, not light tested, 25x25mm, thickness 0.003mm, 99.85%
GF21950197
Iron, tube, 1000mm, outside diameter 3.18mm, inside diameter 1.96mm, wall thickness 0.61mm, as drawn, 99.5%
GF16157190
Iron, foil, 10mm disks, thickness 0.009mm, 99.85%
GF27511781
Iron, wire reel, 5m, diameter 0.25mm, annealed, 99.99+%
GF34071566
Iron, wire reel, 50m, diameter 0.25mm, hard, 99.5%
GF05454132
Iron, wire reel, 1m, diameter 0.5mm, as drawn, 99.95%
GF45366239
Iron, wire reel, 0.1m, diameter 1.0mm, as drawn, 99.99+%
GF29558582
Iron, foil, 50x50mm, thickness 2mm, as rolled, 99.99+%
GF55306964
Iron, wire reel, 5m, diameter 0.5mm, hard, 99.99+%
GF34984659
Iron, foil, 300x300mm, thickness 0.125mm, hard, 99.5%
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Iron, foil, light tested, 300x300mm, thickness 0.075mm, hard, 99.5%
GF04889751
Iron, wire reel, 0.2m, diameter 0.5mm, as drawn, 99.998%
GF45478604
Iron, tube, 200mm, outside diameter 6.35mm, inside diameter 5.35mm, wall thickness 0.5mm, as drawn, 99.8%
GF20981358
Iron, rod, 50mm, diameter 12.7mm, as drawn, 99.95%
GF53990253
Iron, foil, 8mm disks, thickness 0.5mm, hard, 99.5%