Cloning and Expression

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Genotypes of Selected E. coli strains in molecular biology

A genotype is a list of mutant genes in an organism. In addition to mutations of the genome, other genetic elements such as prophage or plasmids can also be included.

Cloning & Expression Vectors

Expression Vectors for Bacterial, Mammalian and Insect Cell Systems. Featured are a variety of tags, promoters and elements for secretion, transient, stable and bicistronic expression.

Reverse Transfection of Plasmid DNA

Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

T7 Promoter System

Bacterial Expression Vectors: T7 Promoter System. T7 Vectors for Highest Expression Levels in Bacteria.

Blue-White Screening & Protocols for Colony Selection

Learn about and find protocols for the blue white screen technique used in molecular biology research to identify recombinant bacterial clones for further analysis.

Blue-White Screening & Protocols for Colony Selection

Learn about and find protocols for the blue white screen technique used in molecular biology research to identify recombinant bacterial clones for further analysis.

Cloning Genes-of-Interest into a Plasmid Vector

This cloning protocol includes selecting the cloning system and plasmid vector, plasmid restriction digestion, fragment restriction digestion, gel excision, dephosphorylating DNA and more.

Cloning Genes-of-Interest into a Plasmid Vector

This cloning protocol includes selecting the cloning system and plasmid vector, plasmid restriction digestion, fragment restriction digestion, gel excision, dephosphorylating DNA and more.

Competent Cell Selection & E. coli Markers Guide

Select competent cells by application, transformation efficiency, and genotype. We also present a list of E. coli markers for reference.

FLAG® Tag Bacterial and Mammalian Expression Vectors

FLAG® and 3xFLAG® expression vectors and products for bacterial and mammalian expression vectors, detection and purification of proteins.

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

FLAG® Tag Bacterial and Mammalian Expression Vectors

FLAG® and 3xFLAG® expression vectors and products for bacterial and mammalian expression vectors, detection and purification of proteins.

3xFLAG® System Expression Vectors for Ultra-Sens

The 3xFLAG system enhances fusion protein detection, offering improved sensitivity over traditional methods.

3xFLAG® System Expression Vectors for Ultra-Sens

The 3xFLAG system enhances fusion protein detection, offering improved sensitivity over traditional methods.

Cloning Troubleshooting

Molecular cloning inserts genes into plasmid vectors for protein expression in different studies.

Cloning Troubleshooting

Molecular cloning inserts genes into plasmid vectors for protein expression in different studies.

Cloning Troubleshooting

Molecular cloning inserts genes into plasmid vectors for protein expression in different studies.

Yeast Transformation Introduction

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

Synthesis of Polyamino Acids

Polyamino acids adopt ordered conformations with unique biological properties and functions like α-helices and β-sheets.

Synthesis of Polyamino Acids

Polyamino acids adopt ordered conformations with unique biological properties and functions like α-helices and β-sheets.

SnapFast™ Restriction Site Functions

SnapFast™ plasmid system eliminates restriction sites in DNA sections, ensuring flexibility and functionality in molecular cloning..

SnapFast™ Restriction Site Functions

SnapFast™ plasmid system eliminates restriction sites in DNA sections, ensuring flexibility and functionality in molecular cloning..

Plasmid Product Nomenclature

SnapFast system offers versatile plasmid cloning with pre-designed DNA sections for easy incorporation or transfer between plasmids.

Plasmid Product Nomenclature

SnapFast system offers versatile plasmid cloning with pre-designed DNA sections for easy incorporation or transfer between plasmids.

Plasmid Product Nomenclature

SnapFast system offers versatile plasmid cloning with pre-designed DNA sections for easy incorporation or transfer between plasmids.

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