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Gene expression and silencing

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facet applications:Gene expression and silencing
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Showing 1-30 of 154 results
CRISPR-based Gene Activation
Sigma has developed a gene activation system based on a fusion of dCas9 to the catalytic histone acetyltransferase (HAT) core domain of the human E1A-associated protein p300.
Reverse Transfection of Plasmid DNA
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways
Presenting an article on Pooled Lentiviral shRNA Screens to Identify Essential Cancer Pathways
siRNA FAQ
Sigma-Aldrich® answers your frequently asked questions about siRNA including experimental design and analysis, advanced applications, custom options and much more.
in vivo FAQs
Sigma-Aldrich® answers your frequently asked questions about in vivo siRNA applications
Lentivirus Cell Line
Using lentivirus as a means to deliver shRNAs has become standard practice in many labs exploring RNAi.
Delivery of Nucleic Acids Using Polymers
This review will focus on the sub-sector of drug delivery concerning the delivery of nucleic acids, with a particular focus on poly(ethyleneimine).
Custom Services
We are the exclusive distributor of the TRC shRNA library clones in DNA or lentiviral format and many custom services are available.
Development of a New Advanced KSOM Mouse Embryo Media that Enables Dual-Use Embryo Culture and Cell Handling Applications
Advanced KSOM mouse embryo media that can be used as a single medium for both harvest and culture of mouse embryos to facilitate creation of transgenic knockout mice.
Successful Transduction Using Lentivirus
Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
Oligonucleotide Quality Control & Quality Assurance
The foundation of Sigma's manufacturing process is a robust QMS, which drives compliance to ISO 9001:2008 certification.
Oligonucleotide Quality Control & Quality Assurance
The foundation of Sigma's manufacturing process is a robust QMS, which drives compliance to ISO 9001:2008 certification.
Chromatin Immunoprecipitation (ChIP) Assay
Use chromatin immunoprecipitation (ChIP) to detect and relatively quantify specific protein-DNA and protein-protein interactions in vivo at a single locus or multiple loci.
Glycosaminoglycan Sulfation and Signaling
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
Glycosaminoglycan Sulfation and Signaling
Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.
siRNA Delivery in Mammalian Cell Lines
siRNAs are effectively delivered into mammalian cell lines that have been difficult or even impossible to transfect with siRNA when using other commercially available lipofection reagents.
siRNA Delivery in Mammalian Cell Lines
siRNAs are effectively delivered into mammalian cell lines that have been difficult or even impossible to transfect with siRNA when using other commercially available lipofection reagents.
shRNA Process and Diagram
When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
Experimental Design and Analysis — Experimental Design FAQs
We answer your frequently asked questions about designing shRNA experiments, including appropriate controls, screening techniques, selection and more.
Experimental Design and Analysis — Experimental Design FAQs
We answer your frequently asked questions about designing shRNA experiments, including appropriate controls, screening techniques, selection and more.
Experimental Design and Analysis — Experimental Design FAQs
We answer your frequently asked questions about designing shRNA experiments, including appropriate controls, screening techniques, selection and more.
Experimental Design and Analysis — Experimental Design FAQs
We answer your frequently asked questions about designing shRNA experiments, including appropriate controls, screening techniques, selection and more.
MISSION® FAQs
We answer your frequently asked questions about shRNA including available formats, experimental design and analysis, advanced applications, ordering, custom options and much more.
MISSION® FAQs
We answer your frequently asked questions about shRNA including available formats, experimental design and analysis, advanced applications, ordering, custom options and much more.
esiRNA FAQs (Frequently Asked Questions)
esiRNA are endoribonuclease prepared siRNAs that target the same mRNA sequence for gene silencing. Here are some of the most asked questions regarding esiRNA uses and availability.
esiRNA FAQs (Frequently Asked Questions)
esiRNA are endoribonuclease prepared siRNAs that target the same mRNA sequence for gene silencing. Here are some of the most asked questions regarding esiRNA uses and availability.
General shRNA FAQs
We answers your frequently asked questions about shRNA, the TRC. MISSION and more.
General shRNA FAQs
We answers your frequently asked questions about shRNA, the TRC. MISSION and more.
General shRNA FAQs
We answers your frequently asked questions about shRNA, the TRC. MISSION and more.
General shRNA FAQs
We answers your frequently asked questions about shRNA, the TRC. MISSION and more.
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