803391
SM(PEG)24 (PEGylated, long-chain SMCC crosslinker)
Synonym(s):
PEGylated SM(PEG)24
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About This Item
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form
powder
Quality Level
mol wt
1394.55
reaction suitability
reagent type: cross-linking reagent
storage condition
desiccated
shipped in
ambient
storage temp.
−20°C
SMILES string
O=C(N1OC(CCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCNC(CCN2C(C=CC2=O)=O)=O)=O)CCC1=O
Related Categories
General description
SM(PEG)n NHS- and maleimide-activated PEG compounds for crosslinking between primary amines (NH2) and sulfhydryl (SH) groups in proteins and other molecules. A complete set of compounds are offered, each having the same heterobifunctional structure (NHS-PEGn-Maleimide) but differing in the number of discrete ethylene glycol units ( n = 2, 4, 6, 8, 12 or 24). The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds. The maleimide group reacts with reduced sulfhydryls at pH 6.5-7.5 to form stable thioether bonds
Application
SM(PEG)24 can be used as a crosslinker in:
- The study of chemical cross-linking of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and RNAses using the high-mass MALDI-MS technique.
- Preparation of protein G-liposomal nanovesicles applicable as sensors in the detection of gentamycin in the milk sample.
- Conjugation of catalase to streptavidin, which is helpful in the detection of analytes at very low concentrations using ELISA technique.
Features and Benefits
- Reactive groups: NHS ester and maleimide
- Reactive toward: amino and sulfhydryl groups
- Irreversibly crosslink proteins or peptides by flexible PEG spacer arms
- Polyethylene glycol spacer arms help maintain conjugate solubility
- Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects
- PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
- Ideal for performing controlled, two-step protein conjugations (see Sulfo-SMCC)
Caution
This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Plasmonic ELISA for the detection of analytes at ultralow concentrations with the naked eye
Nature Protocols, 8(9), 1759-1759 (2013)
Understanding chemical reactivity for homo-and heterobifunctional protein cross-linking agents
Journal of Mass Spectrometry : Jms, 48(7), 807-812 (2013)
Development of a novel bead-based 96-well filtration plate competitive immunoassay for the detection of Gentamycin
Biosensors And Bioelectronics, 49(7), 126-132 (2013)
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