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Merck

803537

Bis(NHS)PEG5

Synonym(s):

Bis-N-succinimidyl-(pentaethylene glycol) ester, Bis-succinimide ester-activated PEG compound

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100 MG

MXP 10,064.00

MXP 10,064.00

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About This Item

Empirical Formula (Hill Notation):
C22H32N2O13
CAS Number:
756526-03-1
Molecular Weight:
532.50
MDL number:
MFCD11041118
UNSPSC Code:
12352200
NACRES:
NA.22
Form:
liquid

Key Documents

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InChI key

FTYUGLBWKRXQBD-UHFFFAOYSA-N

SMILES string

N2(C(=O)CCC2=O)OC(=O)CCOCCOCCOCCOCCOCCC(=O)ON1C(=O)CCC1=O

form

liquid

mol wt

532.5

reaction suitability

reagent type: cross-linking reagent

storage condition

desiccated

solubility

water: soluble

shipped in

ambient

storage temp.

−20°C

Quality Level

100

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This Item
803464713783JKA5078
Bis(NHS)PEG5

Sigma-Aldrich

803537

Bis(NHS)PEG5

BS(PEG)9

Sigma-Aldrich

803464

BS(PEG)9

O,O′-Bis[2-(N-Succinimidyl-succinylamino)ethyl]polyethylene glycol 2,000

Sigma-Aldrich

713783

O,O′-Bis[2-(N-Succinimidyl-succinylamino)ethyl]polyethylene glycol

HO-PEG5K-NHS average Mn 5,000

Sigma-Aldrich

JKA5078

HO-PEG5K-NHS

solubility

water: soluble

solubility

water: soluble

solubility

-

solubility

-

reaction suitability

reagent type: cross-linking reagent

reaction suitability

reagent type: cross-linking reagent

reaction suitability

reagent type: cross-linking reagent

reaction suitability

reagent type: cross-linking reagent
reactivity: amine reactive

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

-

form

liquid

form

oil

form

powder

form

solid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

532.5

mol wt

708.71

mol wt

average Mn 2,000

mol wt

average Mn 5,000

General description

By contrast to typical PEG reagents that contain heterogeneous mixtures of different PEG chain lengths, this PEG reagent is a homogeneous compound of defined molecular weight and spacer arm length, providing greater precision in optimization and characterization of crosslinking applications. Homobifunctional crosslinkers containing N-hydroxysuccinimide (NHS) esters are often used for low-resolution 3-D studies of protein structure and protein interaction analysis.

Features and Benefits

  • Reactive groups: NHS ester (both ends)
  • Reactive towards: amino groups (primary amines)
  • NHS esters react with—NH2 groups at pH 7-9, forming stable amide bonds
  • Water-soluble; compare to non-pegylated BS3 (also soluble) and DSS (water-insoluble)
  • Membrane-impermeable, allowing for cell surface labeling
  • Irreversibly crosslink proteins or peptides by flexible PEG spacer arms
  • Polyethylene glycol spacer arms help maintain conjugate solubility
  • Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects
  • PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity
  • Ideal for small molecule or peptide conjugations

Disclaimer

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
WI337107

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G Mattson et al.
Molecular biology reports, 17(3), 167-183 (1993-04-01)
The various aspects of chemical crosslinking are addressed. Crosslinker reactivity, specificity, spacer arm length and solubility characteristics are detailed. Considerations for choosing one of these crosslinkers for a particular application are given as well as reaction conditions and practical tips
S Knoller et al.
The Journal of biological chemistry, 266(5), 2795-2804 (1991-02-15)
The superoxide (O2-) forming NADPH oxidase complex of resting phagocytes can be activated in a cell-free system by certain anionic amphiphiles, such as sodium dodecyl sulfate (SDS). For O2- production to occur, the participation of both membrane-associated and cytosol-derived components
G W Cox et al.
Journal of immunology (Baltimore, Md. : 1950), 145(6), 1719-1726 (1990-09-15)
This study was designed to examine the expression and function of IL-2R on murine macrophages. We used a model system of murine macrophage cell lines (ANA-1 and GG2EE) that was established by infecting normal murine bone marrow-derived cells with the

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