356352-M

Glycerol, Molecular Biology Grade - CAS 56-81-5 - Calbiochem

• Synonym(s): Glycerol, 1,2,3-Propanetriol, Glycerin
• Linear Formula: HOCH₂CH(OH)CH₂OH
• CAS Number: 56-81-5
• Molecular Weight: 92.09
• Beilstein: 635685
• MDL number: MFCD00004722

Properties

• vapor density: 3.1 (vs air)
• Quality Level: 100
• vapor pressure: <1 mmHg ( 20 °C)
• assay: ≥99% (titration)
• form: liquid
• autoignition temp.: 698 °F
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated
• technique(s): electrophoresis: suitable
• refractive index: n20/D 1.474 (lit.)
• bp: 182 °C/20 mmHg (lit.)
• mp: 20 °C (lit.)
• density: 1.25 g/mL (lit.)
• cation traces: heavy metals: ≤0.0005%
• absorption: ≤0.02 at 280 nm in H₂O at 0.5 M; ≤0.07 at 260 nm in H₂O at 0.5 M
• foreign activity: DNases, proteases, RNases, none detected
• storage temp.: 10-30°C
• SMILES string: OCC(O)CO
• InChI: 1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2
• InChI key: PEDCQBHIVMGVHV-UHFFFAOYSA-N

Description

General description

Obtained from oils and fats as a biproduct in the manufacturing of soaps and fatty acids (absorbance: ≤0.07 at 260 nm; ≤0.02 at 280 nm).

Application

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Warning

Toxicity: Standard Handling (A)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 390.2 °F - Pensky-Martens closed cup
• Flash Point(C): 199 °C - Pensky-Martens closed cup