SCC300

3dGRO^™ Human iPSC Derived Colon Organoids

• Synonym(s): Human Colon Organoids, Human Colonoids, Human Gut Organoids, Human Intestinal Organoids

Properties

• form: liquid
• technique(s): cell culture | stem cell: suitable
• application(s): cell analysis

Description

General description

Human intestinal organoids are self-organizing, 3D structures that can be expanded in long-term culture and differentiate into intestinal epithelial cell types. Epithelial intestinal organoids, often referred to as enteroids or “mini-guts”, maintain the physiological characteristics of the gastrointestinal system and have been a useful cell culture tool to model intestinal development and disease including the study of colon cancer, celiac disease, inflammatory bowel diseases and host microbiome interactions. Organoids can be propagated from patient biopsies and from embryonic (ES) and induced pluripotent stem cells (iPS cells).

3dGRO^™ Human iPSC Derived Colon Organoids are derived from integration-free human iPS cells (Cat. No. SCC271) reprogrammed using the Simplicon RNA reprogramming technology (Cat. No. SCR550). Human iPS cells were differentiated using a multi-step process from definitive endoderm to posterior hind-gut endoderm and eventually into colon organoids (Figure 1.). The organoids express colon-specific markers including the posterior hindgut marker CDX2, a-carbonic anydrase II (CA-II), a-carbonic anhydrase IV (CA-IV), and goblet cell markers Mucin-2 and Mucin-5B. 3dGRO^™ human iPSC derived colon organoids can be propagated long-term by passaging every 10-12 days using 3dGRO^™ Human Colon Organoid Expansion Medium (Cat. No. SCM304).

Application

Highly characterized cryopreserved human colon intestinal organoids derived from integration-free human induced pluripotent stem cells (iPSCs).

Research Category:

• Cell Culture
• Stem Cell Research

Quality

• Each vial contains ≥1500 organoids.
• Mycoplasma: Negative
• Genotyped by STR analysis: Pass
• Human species verified
• Organoids are negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
• Cell viability test: Pass

Storage and Stability

3dGRO^™ Human iPSC Derived Colon Organoids should be stored in liquid nitrogen.

Legal Information

3dGRO is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash