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SCM045

Sigma-Aldrich

Human Mesenchymal-XF Expansion Medium

Xeno-free mesenchymal stem cell media specially formulated for the rapid expansion of human mesenchymal stem cells isolated from a variety of sources, including adipose and bone marrow tissues.

eCl@ss:
32160801
NACRES:
NA.75

Quality Level

form

liquid

manufacturer/tradename

Chemicon®

application(s)

cell culture | stem cell: suitable

input

sample type mesenchymal stem cell(s)

shipped in

dry ice

General description

The Human Mesenchymal-XF Expansion Medium is a xeno-free medium specially formulated for the rapid expansion of human mesenchymal stem cells isolated from a variety of sources, including adipose and bone marrow tissues. The medium contains 2% human serum, is free of animal-derived components and supports the growth of human MSCs at rates equal to or greater than other commercial xeno-free/serum-free media and media supplemented with 2-10% FBS. Human MSCs cultured in the medium retain self-renewal and multi-lineage differenitation potential. The Human Mesenchymal-XF Expansion Medium does not contain any antimicrobials, phenol red, or any other components that can cause cell stress and “masking effects” that may influence experimental results.

Application

Research Category
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells
A separate ECM solution is not required to facilitate cell attachment using the human mesenchymal-XF expansion medium.

Components

All components used within the media are of human origin and do not contain any animal derived components.

Quality

These products are manufactured with the highest quality of raw materials, with exacting standards and production procedures to ensure lot-to-lot consistency. Every lot of medium is extensively tested for the following parameters:

Sterility Testing: Negative for bacterial and fungal growth
pH: 7.5 ± 0.3
Cell Testing: Rate of proliferation and morphology
Osmolality: 255 ± 15 mOsm
Endotoxin: <0.5 EU/mL

Physical form

The Human Mesenchymal-XF Expansion Medium is a complete medium and contains all the necessary growth factors and supplements to support human mesenchymal stem cell growth.

Storage and Stability

Frozen medium should be stored at -20ºC until expiration date.
Thawed medium can be stored at 2 to 8ºC for up to 1 month.
Multiple freeze/thaw cycles are not recommended

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Articles

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

Mesenchymal Stem Cell FAQs

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Protocols

Mesenchymal Stem Cell Culture Protocols

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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