Merck
All Photos(1)

10220647001

Roche

GTP-γ-S

Tetralithium salt

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
GTP-γ-S, GTP

description

C10H12N5O13P3SLi4

Quality Level

form

solid

mol wt

Mr 539.2 (GTP-γ-S)
Mr 563.0 (GTP-γ-S-Li4)

composition

GDP, ≤12% HPLC
GMP, ≤1% HPLC
GTP, ≤2% HPLC

packaging

pkg of 10 mg

manufacturer/tradename

Roche

storage condition

protect from light

technique(s)

activity assay: suitable

storage temp.

−20°C (−15°C to −25°C)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
111623060011114096500110106399001
GTP-γ-S Tetralithium salt

Roche

10220647001

GTP-γ-S

ATP-γ-S =90% (HPLC), solution, pkg of 200 μL (20 μmol)

Roche

11162306001

ATP-γ-S

ATP Lithium salt

Roche

11140965001

ATP

GTP >90% (HPLC), crystals, pkg of 250 mg

Roche

10106399001

GTP

mol wt

Mr 539.2 (GTP-γ-S), Mr 563.0 (GTP-γ-S-Li4)

mol wt

523.2

mol wt

-

mol wt

Mr 523.2 (GTP), Mr 567.1 (GTP-Na2)

packaging

pkg of 10 mg

packaging

pkg of 200 μL (20 μmol)

packaging

pkg of 400 μL (4,000 cm2 membrane)

packaging

pkg of 250 mg

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

storage temp.

−20°C (−15°C to −25°C)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

General description

Guanosine 5′-O-(3-thiotriphosphate), tetralithium salt (GTP-γ-S) is a non-hydrolysable analog of GTP. It is an inhibitor of phosphodiesterase in photoreceptors.

Application

GTP-γ-S has been used:
  • in G-Protein activation assay to assess the functionality of protease-activated receptor 4 (PAR4)
  • in fluorimetric guanine nucleotide exchange assay of G protein alpha subunits (Gα)
  • to monitor the Rac family small guanosine triphosphatase (GTPase) 2 (Rac2)-stimulated activity of phospholipase Cγ2 mutants

Biochem/physiol Actions

Guanosine 5′-O-(3-thiotriphosphate) (GTP-γ-S) activates guanine-nucleotide-binding proteins and is slowly hydrolyzed enzymatically. It inhibits guanosine triphosphatase (GTPases) more potently than guanosine triphosphate (GTP),

Storage and Stability

Store powder dry at -15 to -25 °C; aqueous solutions with pH approx. 7 should be stable for six months at -15 to -25 °C.

Analysis Note

Absorption: Content is measured in aqueous solution by absorption at 254 nm.
Conditions:
  • approx. 10 mg/ml double-dist. water
  • dilution: 9.8 ml double-dist. water + 0.2 ml sample.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Claudia Walliser et al.
The Journal of biological chemistry, 291(42), 22136-22148 (2016-08-21)
Mutations in the gene encoding phospholipase C-γ2 (PLCγ2) have been shown to be associated with resistance to targeted therapy of chronic lymphocytic leukemia (CLL) with the Bruton's tyrosine kinase inhibitor ibrutinib. The fact that two of these mutations, R665W and
Stephen R Sprang
Biopolymers, 105(8), 449-462 (2016-03-22)
This review addresses the regulatory consequences of the binding of GTP to the alpha subunits (Gα) of heterotrimeric G proteins, the reaction mechanism of GTP hydrolysis catalyzed by Gα and the means by which GTPase activating proteins (GAPs) stimulate the
S Nawy et al.
Neuron, 7(4), 677-683 (1991-10-01)
Transmitter release from photoreceptors is decreased by light, resulting in a conductance increase in depolarizing bipolar cells. Addition of exogenous cGMP through a patch pipette to depolarizing bipolar cells from slices of dark-adapted tiger salamander retina resulted in an enhancement
K Sasaki et al.
Nature, 325(6101), 259-262 (1987-01-15)
Recently, a GTP-binding protein sensitive to islet activating protein (IAP) has been suggested to be important in producing K+-currents when the muscarinic receptor of the atrial muscle is activated by acetylcholine (ACh). Here we confirm the blocking effects of IAP
Gilbert Di Paolo, Markus R. Wenk
Lipids, 3-18 (2012)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service