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10799050001

Roche

BM-Cyclin

lyophilized, active toward mycoplasma

Quality Level

form

lyophilized

packaging

pkg of 37.5 mg (for 2 x 2.5 l medium)

Mode of action

protein synthesis | interferes

antibiotic activity spectrum

mycoplasma

shipped in

wet ice

storage temp.

2-8°C

General description

BM-Cyclin is a combination of two antibiotics, namely tiamulin and minocycline, both of which prevent protein synthesis.

Specificity

Sensitive Organisms: Mycoplasmas and bacteria

  • BM Cyclin was found to effectively eliminate Acholesplasma laidlawii,
  • Mycoplasma arginini,
  • Mycoplasma hyorhinis, and
  • Mycoplasma orale
from experimentally contaminated and chronically infected cell lines.
Note: These mycoplasma strains account for more than 85% of the contaminations in animal cell cultures.

Application

BM-Cyclin is used for the elimination of mycoplasma from infected cell cultures without obvious marked cytotoxic side effects.

Packaging

Set of two different antibiotics

Physical form

Vial 1 (Tiamulin-hydrogenfumarat): clear, colorless solution after reconstitution
Vial 2 (Minocyclinhydrochloride): clear, yellow solution after reconstitution

Preparation Note

Working concentration: 10μg/ml pleuromutilin derivative (BM-cyclin 1), 5μg/ml tetracycline derivative (BM-cyclin 2).
Note: These concentrations do not affect the growth of most cells. Lower concentrations may be used for sensitive cell lines.
For hybridomas decrease concentration of BM-Cyclin by 50%.
Preparation of DAPI Working Solution
Dilute the stock solution with methanol to a final concentration of 1μg/ml.
Working solution: Preparation of Stock Solution
Dissolve content of BM Cyclin 1 and BM Cyclin 2 in 10ml sterile PBS or water.
Note: These are 250x concentrated stock solutions and can be stored at -15 to -25°C for at least 6 months.Preparation of DAPI Stock Solution
Dissolve DAPI in water to a final concentration of 1 to 5mg/ml.
Note: Do not use any buffers.
This stock solution can be stored at -15 to -25°C. It is recommended to prepare appropriate aliquots.
Storage conditions (working solution): -15 to -25°C
Reconstituted stable at -15 to -25°C for at least 6 months.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Kit Components Only

Product No.
Description

  • BM-Cyclin 1: pleuromutilin derivative

  • BM-Cyclin 2: tetracycline derivative

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Hsuan Jung et al.
Chang Gung medical journal, 26(4), 250-258 (2003-07-09)
Mycoplasmas, the smallest and simplest prokaryotes that reside in endosomes of mammalian cells, are widespread contaminants found in cell cultures. About 30% of all cell cultures, varying from 15 to 80%, are reportedly contaminated with mycoplasmas. Here, we present our...

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