10843555001
Roche
Hygromycin B
from Streptomyces hygroscopicus
Synonym(s):
Hygromycin B from Streptomyces hygroscopicus
About This Item
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biological source
Streptomyces hygroscopicus
sterility
non-sterile; 0.2 μm filtered
Assay
80% (HPLC)
form
buffered aqueous solution
composition
Hygromycin B, >80% HPLC
packaging
pkg of 20 mL (1 g)
manufacturer/tradename
Roche
concentration
50 mg/mL
technique(s)
transfection: suitable
SMILES string
CN[C@H]1C[C@@H](N)[C@H](O)[C@@H](O[C@@H]2O[C@H](CO)[C@H](O)[C@@H]3O[C@]4(O[C@H]([C@H](N)CO)[C@H](O)[C@H](O)[C@H]4O)O[C@H]23)[C@@H]1O
InChI
1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6-,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20+/m1/s1
InChI key
GRRNUXAQVGOGFE-XKIAHZFYSA-N
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General description
- Formula: C20H37N3O13
- LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
- Molecular weight: Mr = 527.5
- Working concentration: 50 - 1,000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.
Application
Biochem/physiol Actions
Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.
Quality
Unit Definition
We do not have this information.
Physical form
Preparation Note
Storage and Stability
Other Notes
Signal Word
Danger
Hazard Statements
Hazard Classifications
Acute Tox. 1 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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Articles
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
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