11427857910
Roche
Fluorescein-12-UTP
≥85% (HPLC), solution
Synonym(s):
Fluorescein-12-UTP tetralithium salt, Fluorescein-5(6)-carboxamidocaproyl-(5-[3-aminoallyl]uridine 5′-triphosphate)
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25 μL
MXP 7,826.00
MXP 7,826.00
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25 μL
MXP 7,826.00
About This Item
Empirical Formula (Hill Notation):
C39H41N4O22P3
CAS Number:
Molecular Weight:
1010.68
MDL number:
UNSPSC Code:
41116100
MXP 7,826.00
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Quality Level
Assay
≥85% (HPLC)
form
solution
mol wt
1034.4
packaging
pkg of 25 μL (250 nmol; 10 mM)
manufacturer/tradename
Roche
storage temp.
−20°C
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General description
Quality
Typical analysis: At least 85% Fluorescein-12-UTP (HPLC, area%).
Typical analysis: At least 85% Fluorescein-12-UTP (HPLC, area%).
Fluorescein is bound to uridine triphosphate via an amide linkage. Molecular formula is C39H37N4O22P3Li4.
Application
Fluorescein-12-UTP is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for RNA labeling.[1]
Labeled RNA can be subsequently detected by in situ hybridization and direct fluorescence detection or detection by ELISA using Anti-Fluorescein-AP, Fab fragments or Anti-Fluorescein-POD, Fab fragments.
Labeled RNA can be subsequently detected by in situ hybridization and direct fluorescence detection or detection by ELISA using Anti-Fluorescein-AP, Fab fragments or Anti-Fluorescein-POD, Fab fragments.
Other Notes
For life science research only. Not for use in diagnostic procedures.
For your convenience, fluorescein-UTP is also available in a convenient labeling mix (single solution with conventional NTPs and Fluorescein-12-UTP).
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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