Merck
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11427857910

Roche

Fluorescein-12-UTP

≥85% (HPLC), solution

All Photos(2)
Synonym(s):
Fluorescein-12-UTP tetralithium salt, Fluorescein-5(6)-carboxamidocaproyl-(5-[3-aminoallyl]uridine 5′-triphosphate)
Empirical Formula (Hill Notation):
C39H41N4O22P3
CAS Number:
134367-01-4
Molecular Weight:
1010.68

Quality Level

100

Assay

≥85% (HPLC)

form

solution

mol wt

1034.4

packaging

pkg of 25 μL (250 nmol; 10 mM)

manufacturer/tradename

Roche

storage temp.

−20°C

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This Item
F3651I927146955
Fluorescein-12-UTP ≥85% (HPLC), solution

Roche

11427857910

Fluorescein-12-UTP

Fluorescein 5(6)-isothiocyanate ≥90% (HPLC)

Sigma-Aldrich

F3651

Fluorescein 5(6)-isothiocyanate

5-(Iodoacetamido)fluorescein ≥90% (HPLC)

Sigma-Aldrich

I9271

5-(Iodoacetamido)fluorescein

Fluorescein for fluorescence, free acid

Sigma-Aldrich

46955

Fluorescein

assay

≥85% (HPLC)

assay

≥90% (HPLC)

assay

≥90% (HPLC)

assay

-

form

solution

form

powder

form

powder

form

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

mol wt

1034.4

mol wt

-

mol wt

-

mol wt

-

packaging

pkg of 25 μL (250 nmol; 10 mM)

packaging

-

packaging

-

packaging

-

General description

Quality
Typical analysis: At least 85% Fluorescein-12-UTP (HPLC, area%).
Fluorescein is bound to uridine triphosphate via an amide linkage. Molecular formula is C39H37N4O22P3Li4.

Application

Fluorescein-12-UTP is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for RNA labeling.
Labeled RNA can be subsequently detected by in situ hybridization and direct fluorescence detection or detection by ELISA using Anti-Fluorescein-AP, Fab fragments or Anti-Fluorescein-POD, Fab fragments.

Other Notes

For life science research only. Not for use in diagnostic procedures.
For your convenience, fluorescein-UTP is also available in a convenient labeling mix (single solution with conventional NTPs and Fluorescein-12-UTP).

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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