11685619910

Fluorescein RNA Labeling Mix

solution, suitable for Northern blotting, suitable for Southern blotting, suitable for hybridization

• Synonym(s): RNA labeling

Properties

• form: solution
• Quality Level: 100
• usage: sufficient for 20 reactions
• packaging: pkg of 40 μL
• manufacturer/tradename: Roche
• technique(s): Northern blotting: suitable; Southern blotting: suitable; hybridization: suitable
• storage temp.: −20°C

Description

General description

Fluorescein RNA Labeling Mix is a convenient nucleotide mixture for the labeling of RNA with fluorescein-12-uridine triphosphate (UTP).

Specificity

Heat inactivation: Stop the reaction by adding 2μl 0.2M EDTA (pH 8.0).

Application

Fluorescein-labeled RNA has been used in a variety of hybridization techniques such as:

• in situ hybridization to tissues
• Southern blots
• northern blots
• plaque or colony lifts
• RNase protection experiments

Quality

The mix is function tested in combination with the DIG RNA Labeling Kit.

Principle

Fluorescein-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Fluorescein-12-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The Fluorescein RNA Labeling Mix is specifically designed for use with the RNA polymerases SP6, T7, and T3, which are supplied with an optimized transcription buffer.

Preparation Note

10x concentrated NTP labeling mixture: 10mM ATP, 10mM CTP, 10mM GTP, 6.5mM UTP, 3.5mM Fluorescein-12-UTP, pH 7.5 (+20°C).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): No data available
• Flash Point(C): No data available