11796828001
Roche
High Pure PCR Template Preparation Kit
pkg of 100 purifications, suitable for DNA extraction
Recommended Products
usage
sufficient for 100 purifications
Quality Level
manufacturer/tradename
Roche
packaging
pkg of 100 purifications
technique(s)
DNA extraction: suitable
Related Categories
General description
High Pure PCR Template Preparation Kit; Instructions For Use
Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants.
Note: A special Inhibitor Removal Buffer is included which allows the use of heparinized sample material (100 U/mL of heparin). This buffer increases the sensitivity and reproducibility of assays performed with the isolated nucleic acid, even when the sample contains heparin.
Capacity: The High Pure Spin Filter Tubes hold up to 700 μL sample volume.
Application
- Long-template PCR
- Real-time, quantitative PCR
- SNP detection
- Southern blotting
- Cloning
Features and Benefits
- Minimize DNA loss using a kit that removes contaminants without precipitation or other handling steps that degrade DNA.
- Recover high molecular weight DNA (30 to 50 kb) that is suitable for long-template PCR.
- Improve reliability and reproducibility in downstream applications (real-time, quantitative PCR).
- Save time and maximize flexibility by preparing multiple PCR templates simultaneously.
- Eliminate the use of hazardous organic compounds such as cesium chloride, phenol, chloroform, and ethidium bromide.
Components
- Tissue Lysis Buffer
- Binding Buffer
- Proteinase K, recombinant PCR grade
- Inhibitor Removal Buffer
- Washing Buffer
- Elution Buffer
- High Pure Filter Tubes
- Collection Tubes
Quality
Principle
Legal Information
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 - Acute Tox. 4 Oral - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 Inhalation - Skin Sens. 1 - STOT SE 3
Target Organs
Respiratory system
WGK
WGK 2
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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