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Merck

12013819001

Roche

Anti-HA-Peroxidase, High Affinity

from rat IgG1

Synonym(s):

antibody

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About This Item

UNSPSC Code:
12352203

Key Documents

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biological source

rat

Quality Level

100

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

clone 3F10, monoclonal

form

lyophilized (clear, colorless solution after reconstitution)

packaging

pkg of 25 U (25 μg)

manufacturer/tradename

Roche

isotype

IgG1

epitope sequence

YPYDVPDYA

storage temp.

2-8°C

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This Item
SAB4200119B9183ROAHAHA
Anti-HA-Peroxidase, High Affinity from rat IgG1

Roche

12013819001

Anti-HA-Peroxidase, High Affinity

Monoclonal Anti-FLAG-Peroxidase antibody produced in rat 2-4 mg/mL, clone 6F7, purified immunoglobulin

Sigma-Aldrich

SAB4200119

Monoclonal Anti-FLAG-Peroxidase antibody produced in rat

Anti-HA-Biotin antibody, Mouse monoclonal clone HA-7, purified from hybridoma cell culture

Sigma-Aldrich

B9183

Anti-HA-Biotin antibody, Mouse monoclonal

Anti-HA High Affinity from rat IgG1

Roche

ROAHAHA

Anti-HA High Affinity

clone

clone 3F10, monoclonal

clone

6F7, monoclonal

clone

HA-7, monoclonal

clone

3F10, monoclonal

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

biotin conjugate

conjugate

unconjugated

biological source

rat

biological source

rat

biological source

mouse

biological source

rat

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C, 2-8°C

storage temp.

2-8°C

form

lyophilized (clear, colorless solution after reconstitution)

form

buffered aqueous solution

form

buffered aqueous solution

form

lyophilized

General description

Anti-HA-Peroxidase, High Affinity is a monoclonal antibody to the HA-peptide (clone 3F10), conjugated to peroxidase.

Immunogen

The epitope consists of amino acids 98-106 from the human influenza virus hemagglutinin protein.

Application

  • Use Anti-HA-Peroxidase, High Affinity for the detection of HA-tagged recombinant proteins using: ELISA
  • Western blot[1][2]
Since Anti-HA High Affinity is a rat monoclonal, it is possible to use it in conjunction with murine monoclonals for double labeling.

Biochem/physiol Actions

Anti-HA-Peroxidase, High Affinity (3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein.
This epitope is also recognized in fusion proteins regardless of its position (N-terminal, C-terminal or internal).

Preparation Note

Stabilizers: proteinaceous stabilizers
Working concentration: The working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
  • Dot blot: 50 mU/ml
  • ELISA: 25 mU/ml
  • Western blot: 50 mU/ml

Add 1.0 ml double-distilled water for a final concentration of 25 U/mL.
Rehydrate for 10 min prior to use.

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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Certificates of Analysis (COA)

Lot/Batch Number
63649300
44323100
75254900
71916900
66292500

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