BSAV-RO

Roche

Bovine Serum Albumin Fraction V

from bovine serum

Quality Level

biological source

bovine

assay

≥98.5%

form

lyophilized

mol wt

Mr =68 kDa

packaging

pkg of 1 kg (10735108001)
pkg of 100 g (10735086001)
pkg of 50 g (10735078001)
pkg of 500 g (10735094001)

manufacturer/tradename

Roche

application(s)

ELISA: suitable
Southern blotting: suitable
western blot: suitable

absorption

≤0.4 at 550 nm

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

General description

For standard applications.

Application

Bovine Serum Albumin Fraction V is suitable for standard applications such as:
  • Stabilizer to prevent proteolysis and denaturation of enzymes and other proteins, especially when the proteins are present in low concentrations.
  • Buffer component in immunochemistry, biochemistry, cell biology, or molecular biology
  • Reducing agent
  • Protein blocker for saturation of protein binding sites in ELISAs, Southern blots, and western blots
  • Media supplement for media that require addition of protein
  • Carrier protein
  • Standard protein in gel electrophoresis and protein determination

Quality

Purity: ≤95% protein, ≤5% H2O; electrophoretically homogeneous
Contaminants: <0.5% Na (flame photometric), <0.006% K (flame photometric), <0.0003% Li (flame photometric), <0.02% Ca (o-cresolphthalein), <0.003% Mg (xylidyl blue), <0.003% heavy metals (as Pb), <0.001% Fe (bathophenanthroline), <0.002% Pi, <0.05% glucose (enzymatic), <0.005% glycerol (enzymatic), <0.025% L-lactate (enzymatic)

Preparation Note

Storage conditions (working solution): In solution, albumin is stable for about three months at -15 to -25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

Francis K Y Siu et al.
Nature protocols, 3(1), 51-58 (2008-01-15)
Southwestern blotting is used to investigate DNA-protein interactions. The advantage of this technique over other related methods such as electrophoretic mobility shift assay (EMSA) and DNA footprinting is that it provides information regarding the molecular weight of unknown protein factor....
Laurent Berchebru et al.
Journal of microbiological methods, 105, 141-145 (2014-07-20)
Test and evaluation of engineered biothreat agent detection systems ("biodetectors") are a challenging task for government agencies and industries involved in biosecurity and biodefense programs. In addition to user friendly features, biodetectors need to perform both highly sensitive and specific...
Mariko Kuse et al.
The Journal of reproduction and development, 59(4), 346-352 (2013-04-09)
Cortisol (Cr), the most important glucocorticoid (GC), is well known to suppress uterine prostaglandin F2α (PGF) production. However, the details of the regulatory mechanisms controlling the cyclic changes in endometrial PGF production remain unclear. Here we investigated the expression of...

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