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DIUTPS-RO

Roche

Digoxigenin-11-dUTP, alkali-stable

Synonym(s):
digoxigenin

assay

≥85% (HPLC)

Quality Level

form

solution

mol wt

Mr 1090.7

packaging

pkg of 125 μL (11558706910 [1 mM])
pkg of 5 × 125 μL (11570013910 [5x1 mM])
pkg of 25 μL (11093088910 [1 mM])

manufacturer/tradename

Roche

λmax

222 and 292 nm
22430 and 8780

storage temp.

−20°C

General description

Digoxigenin-11-dUTP, alkali-stable is provided in 1 mM tetralithium salt, solution.

Application

Digoxigenin-11-dUTP, alkali-stable has been used to label probes for
fluorescent in situ hHybridization (FISH) and polymerase chain reaction (PCR)
Note: When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will remain attached. It is therefore ideal for use in DIG-labeled DNA that needs to survive exposure to alkaline treatment. However, if you intend to make a DIG-labeled probe that can be removed by alkali denaturation (e.g ., during stripping and reprobing of blots), do not use this alkali-stable preparation. Instead, use the alkali-labile form of DIG-11-dUTP.

Biochem/physiol Actions

Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) replaces deoxythymidine triphosphate (dTTP) in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. DIG-11-dUTP can also replace dTTP in labeling during polymerase chain reaction (PCR) (e.g., with Taq DNA Polymerase), 3′-tailing (with terminal transferase) and cDNA synthesis (e.g., with transcriptor or M-MuLV reverse transcriptase). It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, terminal transferase and reverse transcriptase.

Features and Benefits

Digoxigenin is bound to deoxyuridine triphosphate via an alkali-resistant ether linkage.

Resistance to alkali: The preparation is stable in the presence of 0.1 - 0.5M NaOH at +15 to +25°C.

Quality

Typical analysis: At least 85% DIG-11-dUTP (HPLC, area%).
Function tested by RPL.

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).
Storage conditions (working solution): A decomposition of approx. 5% may occur within 4 weeks at 15 to 25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

SDS

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