HPPCRPKRO
Roche
High Pure PCR Product Purification Kit
sufficient for 250 purifications (11732676001), sufficient for 50 purifications (11732668001), suitable for DNA purification
Recommended Products
usage
sufficient for 250 purifications (11732676001)
sufficient for 50 purifications (11732668001)
Quality Level
manufacturer/tradename
Roche
packaging
pkg of 250 purifications (11732676001)
pkg of 50 purifications (11732668001)
technique(s)
DNA purification: suitable
General description
Capacity: The High Pure SpinFilter Tubes hold up to 700 μl sample volume.
Volume: 100 μl
Typical samples include:
- Products from amplification or cDNA synthesis reactions
- Enzymatically treated DNA
- DNA from agarose slices
- Dilute nucleic acid solutions
- RNA from transcription reactions
Application
- Labeling
- Sequencing
- Cloning
- Restriction enzyme digests
- Alkaline phosphatase treatment
- Kinase reactions
The kit eliminates primers, mineral oil, salts, unincorporated nucleotides, and thermostable DNA polymerases, which may inhibit subsequent enzymatic reactions. It can also be applied to concentrate dilute nucleic-acid solutions. Use one kit for a variety of applications.
The fast and simple High Pure protocols use a tabletop centrifuge to bind, wash, and elute the reaction product down to 10 μl (micro format) in as little as 10 minutes. The procedure conveniently eliminates a concentration step, and is ideal for downstream applications such as labeling, sequencing, cloning, ligation, or amplification using PCR.
Features and Benefits
- Quickly purifies multiple PCR products
- Efficiently recover DNA fragments
- Minimize DNA loss
- Eliminate the use of hazardous organic compounds
Components
- Binding Buffer
- Wash Buffer
- Elution Buffer
- High Pure Spin Filter Tubes (containing glass fiber fleece)
- Collection Tubes
Quality
Preparation Note
Other Notes
Signal Word
Danger
Hazard Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C
Supplementary Hazards
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
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Protocols
Determine the labeling efficiency in terms of μg (expected yield of a standard labeling reaction is 20 μg of DIG labeled RNA per μg linearized template DNA after the DIG RNA labeling reaction).
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