14400

Biotin

≥99.0% (T)

• Synonym(s): D-Biotin, Bios II, Coenzyme R, Vitamin B7, Vitamin H
• Empirical Formula (Hill Notation): C₁₀H₁₆N₂O₃S
• CAS Number: 58-85-5
• Molecular Weight: 244.31
• NACRES: NA.21
• PubChem Substance ID: 329750287
• eCl@ss: 34058011
• UNSPSC Code: 12352201
• EC Number: 200-399-3
• MDL number: MFCD00005541
• Beilstein/REAXYS Number: 86838

Properties

• InChI key: YBJHBAHKTGYVGT-ZKWXMUAHSA-N
• InChI: 1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1
• SMILES string: [H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2
• biological source: synthetic
• assay: ≥99.0% (T)
• form: powder
• optical activity: [α]20/D +91±2°, c = 1% in 0.1 M NaOH
• ign. residue: ≤0.1% (as SO₄)
• loss: ≤0.5% loss on drying
• color: colorless to white
• mp: 229-234 °C, 231-233 °C (lit.)
• solubility: alcohol: soluble 80mg/100 ml at 25 °C, water: soluble 22mg/100 ml at 25 °C, 0.1 M NaOH: 10 mg/mL at 25 °C, clear, colorless, H₂O: very soluble, organic solvents: insoluble
• cation traces: Ca: ≤50 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤10 mg/kg, K: ≤50 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Na: ≤500 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg
• application(s): detection
• Quality Level: 200

Description

General description

Biotin is readily available in several foods, like whole grains, nuts, legumes, unpolished rice and egg yolk. The normal gut flora also produces this vitamin.[1]

Application

Biotin has been used for testing the functionality of the Avidin-displaying phages.[2] The product has also been used for labeling the swine anti-rabbit IgG antibody and peroxidase.[3]

Biochem/physiol Actions

Biotin acts as an important cofactor for carboxylase enzymes in various metabolic pathways. Biotin favors keratin synthesis and hence is used as a supplement for hair and nail growth. Deficiency of biotin shows several symptoms such as alopecia, conjunctivitis, seborrheic dermatitis, depression, lethargy, hypotonia and seizures.[1]

Biotin also referred to as vitamin H or vitamin B7 is a water-soluble B-vitamin that induces two to three fold increase in guanylate cyclase activity in rat liver, kidney, colon, cerebellum, and heart.[4] It also stimulates the sperm motility and prolongs the survival of cryopreserved human spermatozoa.[5]

Other Notes

Reviews[6][7]; Avidin-Biotin technology;

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)