Merck
All Photos(3)

252859

Sigma-Aldrich

Tris(hydroxymethyl)aminomethane

ACS reagent, ≥99.8%

Synonym(s):
Tris base, Trometamol, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

grade

ACS reagent

assay

≥99.8%
99.8-100.1%

form

crystalline

impurities

≤0.005% insolubles
≤2% water

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

cation traces

Fe: ≤5 ppm
heavy metals: ≤5 ppm (ICP)

absorption

passes test

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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General description

Tris(hydroxymethyl)aminomethane is mainly required for preparation of buffers at physiological range of 7.3 to 7.5. The prepared buffers are compatible with biological fluids. It is of importance to laboratories as a standard pH solution.

Application

Tris(hydroxymethyl)aminomethane has been used as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.

Packaging

100, 500 g in poly bottle

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

An optimized lactate dehydrogenase release assay for screening of drug candidates in neuroscience.
Kaja S et al.
Journal of Pharmacological and Toxicological Methods, 73, 1-1 (2015)
Tris-tris-HCl: a standard buffer for use in the physiologic pH range.
R A Durst et al.
Clinical chemistry, 18(3), 206-208 (1972-03-01)
Fernando E Díaz-Manzano et al.
Frontiers in plant science, 7, 966-966 (2016-07-28)
Galls induced by Meloidogyne spp. in plant roots are a complex organ formed by heterogeneous tissues; within them there are 5-8 giant cells (GCs) that root-knot nematodes use for their own nurturing. Subtle regulatory mechanisms likely mediate the massive gene
Jianbin Zhang et al.
Autophagy, 10(5), 901-912 (2014-03-29)
Autophagy is a catabolic process during which cellular components including protein aggregates and organelles are degraded via a lysosome-dependent process to sustain metabolic homeostasis during nutrient or energy deprivation. Measuring the rate of proteolysis of long-lived proteins is a classical
Corey A Baron et al.
Magnetic resonance in medicine, 73(3), 1075-1084 (2014-04-12)
An acquisition method that does not increase scan time or specific absorption rate is investigated for reducing the deleterious effects of cerebrospinal fluid (CSF) partial volume effects on diffusion tensor imaging (DTI) tractography. It is based on using a shorter

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