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Sigma-Aldrich

Tris(hydroxymethyl)aminomethane

ACS reagent, ≥99.8%

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Synonym(s):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

ACS reagent

Quality Level

Assay

≥99.8%
99.8-100.1%

form

crystalline

technique(s)

affinity chromatography: suitable

impurities

≤0.005% insolubles
≤2% water

color

white

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

cation traces

Fe: ≤5 ppm
heavy metals: ≤5 ppm (ICP)

absorption

passes test

application(s)

clinical research
diagnostic assay manufacturing
microbiology

foreign activity

DNase, none detected
NICKase, none detected
RNase, none detected
protease, none detected

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

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General description

Tris(hydroxymethyl)aminomethane, commonly known as Trometamol, Tris base, or Trizma base, plays a crucial role in diverse research applications as a biological buffer. It is extensively used in the formulation of buffers like Tris-acetate-EDTA (TAE) and Tris-borate-EDTA (TBE), owing to its optimal pKa of 8.1, making it well-suited for maintaining pH within the physiological range (pH 7 -9) applicable to most living organisms. However, caution might be necessary when studying some proteins, as Tris has the potential to interfere with the activity of certain enzymes. Tris base can be employed both independently as a buffer and as a component in mixed buffer formulations, including Tris-EDTA (TE) buffer, TAE buffer, and TBE buffer, among others. Importantly, Tris base is characterized by its purity, essential stability, and relative non-hygroscopic nature.

In laboratory settings, Tris base is indispensable for the preparation of buffers compatible with biological fluids, acting as a standard pH solution. It facilitates various procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. Its versatility extends to applications in cell culture, biochemistry, and molecular biology labs, where it contributes to studies involving cell membrane permeability and buffer preparation.

Application

Tris(hydroxymethyl)aminomethane has been used:
  • as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.
  • as a buffer in flow cell sample preparation for Raman spectroscopy
  • as a wash buffer in Western blotting
  • as a buffer component in decellularization of tissues

Features and Benefits

  • Efficient buffering within the pH range of 7-9 with a pKa of 8.1 (25 °C)
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Can be used in Cell Biology, and Biochemical research

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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