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Merck

63069

Magnesium chloride solution

BioUltra, Molecular Biology, ~1 M in H2O

Magnesium chloride solution
1 of 1 reviewers received a sample product or took part in a promotion

Synonym(s):

Magnesium Chloride hydrate, Magnesium chloride (MgCl2)hexahydrate, MgCl2

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MXP 3,059.00

500 ML

MXP 12,637.00

MXP 3,059.00

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About This Item

Linear Formula:
MgCl2
CAS Number:
7786-30-3
Molecular Weight:
95.21
NACRES:
NA.26
PubChem Substance ID:
329759772
UNSPSC Code:
12352302
MDL number:
MFCD00011106
Grade:
Molecular Biology
Form:
liquid
Solubility:
water: soluble

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InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

SMILES string

Cl[Mg]Cl

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

grade

Molecular Biology

product line

BioUltra

form

liquid

concentration

~1 M in H2O

technique(s)

HPLC: suitable, flow cytometry: suitable

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.0002% total Nitrogen (N)

pH

5.0-7.5 (25 °C, 1 M in H2O)

solubility

water: soluble

density

1.08 g/mL at 20 °C

anion traces

phosphate (PO43-): ≤10 mg/kg, sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤1 mg/kg, As: ≤0.1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤10 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤50 mg/kg, Li: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Na: ≤500 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.05, λ: 280 nm Amax: 0.03

suitability

suitable for molecular biology

application(s)

cell analysis
filtration
vaccine development

Quality Level

200

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Show Differences

1 of 4

This Item
M878768475M8266
Magnesium chloride solution BioUltra, Molecular Biology, ~1 M in H2O

Sigma-Aldrich

63069

Magnesium chloride solution

Magnesium chloride solution PCR Reagent, 25 mM MgCI2 solution for PCR

Sigma-Aldrich

M8787

Magnesium chloride solution

Magnesium chloride solution BioUltra, Molecular Biology, 2 M in H2O

Sigma-Aldrich

68475

Magnesium chloride solution

Magnesium chloride ≥98%

Sigma-Aldrich

M8266

Magnesium chloride

form

liquid

form

liquid

form

liquid

form

powder

solubility

water: soluble

solubility

-

solubility

-

solubility

-

Quality Level

200

Quality Level

200, 300

Quality Level

200

Quality Level

300

grade

for molecular biology

grade

PCR Reagent

grade

for molecular biology

grade

anhydrous

impurities

DNases, none detected, insoluble matter, passes filter test, proteases, none detected, RNases, none detected, ≤0.0002% total Nitrogen (N), phosphatases, none detected

impurities

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.0002% total nitrogen (N)

impurities

≤0.5% Insolubles

density

1.08 g/mL at 20 °C

density

-

density

-

density

2.32 g/mL at 25 °C (lit.)

Application

Magnesium chloride solution has been used as a component of nuclei isolation buffer for in situ fixations and isolation of nuclei from Kupffer cells (or other liver cells).[1]
Magnesium chloride solution has been used in the reaction mix of reverse transcriptase enzyme, PFV RT.[2] It has been used in the preparation of artificial cerebrospinal fluid (ACSF).[3]

General description

Magnesium chloride is widely used in polymerase chain reactions (PCR) buffers to supply magnesium ions. The concentration of Mg2+ is the most critical parameter that impacts both the efficiency and specificity of PCR when total genomic DNA is used, synthesis of long products, or during multiplex reactions. Elevated concentrations of Mg2+ enhance the stability of primer-template duplexes and the efficiency of amplification.[4]

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN1453
BCCD4296
BCCD4298
BCCM3946
BCCJ0554

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RNase H Polymerase-independent Cleavage Assay for Evaluation of RNase H Activity of Reverse Transcriptase Enzymes.
Corona A and Tramontano E
Bio-protocol null
Ty D Troutman et al.
STAR protocols, 2(1), 100363-100363 (2021-03-23)
Significant advancements in understanding disease mechanisms can occur through combined analysis of next-generation sequencing datasets generated using purified cell populations. Here, we detail our optimized protocol for purification of mouse hepatic macrophages (or other liver non-parenchymal populations) suitable for use
Benjamin Judkewitz et al.
Nature protocols, 4(6), 862-869 (2009-05-16)
In order to link our knowledge of single neurons with theories of network function, it has been a long-standing goal to manipulate the activity and gene expression of identified subsets of mammalian neurons within the intact brain in vivo. This
Hideyuki Arita et al.
Brain tumor pathology, 32(1), 22-30 (2014-04-22)
Assessment of the mutational status of the isocitrate dehydrogenase 1/2 (IDH1/2) gene has become an integral part of the standard diagnostic procedure and, therefore, needs to be accurate. This may, however, be compromised by various factors including the method of
Elaine T Lim et al.
PLoS genetics, 10(7), e1004494-e1004494 (2014-08-01)
Exome sequencing studies in complex diseases are challenged by the allelic heterogeneity, large number and modest effect sizes of associated variants on disease risk and the presence of large numbers of neutral variants, even in phenotypically relevant genes. Isolated populations
View All Related Papers
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Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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1 of 1 reviewers received a sample product or took part in a promotion
1 out of 1 (100%) reviewers recommend this product

Reviews

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  1. Switzerland
    • Reviews 2
    • Votes 0
    5 out of 5 stars.

    Good as ever

    It is now in a plastic bottle, not in the glass anymore but it still works nicely

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