All Photos(8)

B4501

Sigma-Aldrich

Biotin

≥99% (HPLC), lyophilized powder

Synonym(s):
Bios II, Vitamin H, Vitamin B7, D-Biotin, Coenzyme R
Empirical Formula (Hill Notation):
C10H16N2O3S
CAS Number:
Molecular Weight:
244.31
Beilstein:
86838
EC Number:
MDL number:
eCl@ss:
34058011
PubChem Substance ID:
NACRES:
NA.21

Quality Level

biological source

synthetic (organic)

assay

≥99% (HPLC)

form

lyophilized powder

application(s)

HPLC: suitable

color

white to off-white

mp

231-233 °C (lit.)

solubility

ammonium hydroxide: 50 mg/mL (2 M)

storage temp.

2-8°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2

InChI

1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1

InChI key

YBJHBAHKTGYVGT-ZKWXMUAHSA-N

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Application

Biotin may be used to elute proteins from avidin/streptavidin resins.
  • It has been used for culturing of oligodendrocytes.
  • It has been used as a vitamin supplement for the growth of Bacillus species.
  • It has been used for blocking endogenous biotin during immunohistology procedures.

Packaging

100, 500 mg in poly bottle
1, 5, 10, 25 g in poly bottle

Biochem/physiol Actions

Biotin is a small molecule and its presence with biologically active macromolecules does not affect the biological function of the macromolecule. The biotin-(strept)avidin system are required for various applications. Biotin also works as a cofactor of carboxylating enzymes, such as pyruvate carboxylase. It catalyzes the formation of oxaloacetate from pyruvate and CO2. Biotin conjugation with avidin or streptavidin helps in linking the target molecule (antibody, nucleotide, protein A etc.) with the labeling system (enzyme, fluorescent, chemiluminescent probes). This complex is used for many detection systems, such as immunoassays, DNA hybridization assays, immunohistochemistry and flow-cytometry. This approach is also applied for the purification and characterization of various molecules of interest.

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

The biotin-(strept)avidin system: principles and applications in biotechnology.
Diamandis EP and Christopoulos TK
Clinical Chemistry, 37, 625-625 (1991)
Isolation and culture of rat and mouse oligodendrocyte precursor cells.
Chen Y et al.
Nature Protocols, 2, 1044-1044 (2007)
Lone Frydelund-Larsen et al.
Experimental physiology, 92(1), 233-240 (2006-10-13)
Exercise induces a marked increase in interleukin-8 (IL-8) mRNA and protein expression within skeletal muscle fibres. Interleukin-8 belongs to a subfamily of CXC chemokines containing a Glu-Leu-Arg (ELR) motif. CXC chemokines with ELR motifs are potent angiogenic factors in vivo...
Wei Wang et al.
The Journal of cell biology, 219(2) (2020-01-09)
Hemidesmosomes are specialized cell-matrix adhesion structures that are associated with the keratin cytoskeleton. Although the adhesion function of hemidesmosomes has been extensively studied, their role in mechanosignaling and transduction remains largely unexplored. Here, we show that keratinocytes lacking hemidesmosomal integrin...
Anders Rinnov Nielsen et al.
The Journal of physiology, 584(Pt 1), 305-312 (2007-08-11)
The cytokine interleukin-15 (IL-15) has been demonstrated to have anabolic effects in cell culture systems. We tested the hypothesis that IL-15 is predominantly expressed by type 2 skeletal muscle fibres, and that resistance exercise regulates IL-15 expression in muscle. Triceps...

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