C4916

5-Carboxyfluorescein diacetate

~95% (HPLC)

• Synonym(s): 5-CFDA, 5-Carboxy-di-O-acetylfluorescein
• Empirical Formula (Hill Notation): C₂₅H₁₆O₉
• CAS Number: 79955-27-4
• Molecular Weight: 460.39
• Beilstein: 68265
• MDL number: MFCD00036872
• PubChem Substance ID: 24892738
• NACRES: NA.47

Properties

• Assay: ~95% (HPLC)
• Quality Level: 200
• form: powder
• solubility: chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(O)=O)c1
• InChI: 1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)
• InChI key: WPUZGNPQMIWOHE-UHFFFAOYSA-N

Description

Application

5-Carboxyfluorescein diacetate has been used for the staining of bacteria to study bacterial adhesion to mucin.

Biochem/physiol Actions

5-Carboxyfluorescein diacetate is required for the staining of cells to differentiate between live and dead cells. It helps in monitoring integrity and intactness of cell membrane. 5-Carboxyfluorescein diacetate is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product carboxyfluorescein. In viable cells, the cellular membrane is impermeable to the fluorescent molecule.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves