PPB006

Phosphate buffered saline

powder, pH 7.4, pHast Pack^™

• Synonym(s): 1X PBS wash buffer, PBS
• UNSPSC Code: 12161700
• NACRES: NA.21
• MDL number: MFCD00131855

Properties

• Product Name: Phosphate buffered saline, pH 7.4, pHast Pack^™, powder
• product line: pHast Pack^™
• form: powder
• storage condition: (Tightly closed. Dry)
• impurities: <5—2 ppm Heavy metals (Lead)
• color: white
• pH: 7.4
• solubility: soluble, clear, colorless
• cation traces: Fe: <10 ppm
• foreign activity: DNAse, none detected; Nickase, none detected; Protease, none detected; RNAse, none detected
• storage temp.: room temp

Description

Application

Phosphate buffered saline is commonly used in washing solutions for:

• ELISA
• Western Blotting and other protein applications
• Cell culture (sterile filtration recommended)
• Resuspension and sonication of cells
• Immunohistochemistry

Features and Benefits

• Save time with our ready-to-use formulation
• No calculations, measurements, and adjusting pH
• Reliable quality for reproducibility
• Suitable for use in cell culture and electrophoresis
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

General description

Phosphate buffered saline or PBS, pH 7.4 is a commonly used biological buffer in molecular biology. A 1X working solution is typically used in cell culture and electrophoresis assays to wash away non-adherent cells or any unbound biomolecules. PBS is hypo-osmotic and can be harmful to cells, causing swelling and resulting in cell lysis, if left for extended periods.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Packaging

Foil pouches

Preparation Note

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 10mM Na2HPO4, 1.8mM KH2PO4, 138mM NaCl, 2.7mM KCl, pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Legal Information

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable