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09978

Sigma-Aldrich

Ammonium sulfate

BioUltra, ≥99.0% (T)

Synonym(s):
Mascagnite
Linear Formula:
(NH4)2SO4
CAS Number:
Molecular Weight:
132.14
EC Number:
MDL number:
eCl@ss:
38030408
PubChem Substance ID:
NACRES:
NA.26

product line

BioUltra

Quality Level

assay

≥99.0% (T)

form

crystals

impurities

insoluble matter, passes filter test

ign. residue

≤0.01% (as SO4)

loss

≤0.1% loss on drying, 110 °C

pH

5.0-6.0 (25 °C, 1 M in H2O)

mp

>280 °C (dec.) (lit.)

solubility

H2O: 1 M at 20 °C, clear, colorless

density

1.77 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 mg/kg
nitrate (NO3-): ≤50 mg/kg
phosphate (PO43-): ≤5 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.2 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤1 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤2 mg/kg
Fe: ≤2 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤50 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤2 mg/kg
Sr: ≤5 mg/kg
Zn: ≤1 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.037
λ: 280 nm Amax: ≤0.030

SMILES string

N.N.OS(O)(=O)=O

InChI

1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4)

InChI key

BFNBIHQBYMNNAN-UHFFFAOYSA-N

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General description

Ammonium sulfate is naturally present as a rare mineral mascagnite in volcanic fumaroles and due to coal fires in some dumps.

Application

Ammonium sulfate has been used:
  • to prepare mobile phase A for hydrophobic interaction chromatography (HIC)
  • as reference material for the thermal decomposition of NH4NO3 (S1–S6) thermal decomposition to N2O (S1-N2O–S6-N2O)
  • to prepare the stock solution for M63 medium
  • as a reagent in molecular biology and chromatography
  • in the precipitation and fractionation of proteins
  • in the purification of antibodies
  • for the crystallographic analysis of nucleic acids and proteins
  • in high-performance liquid chromatography (HPLC) analysis of proteins, such as in hydrophobic interaction chromatography
  • for Sepharose chromatography of tRNAs using reverse salt gradients
  • in long PCR buffer, in PCR lysis solution, and second-strand cDNA synthesis
  • HPLC analysis of phospholipids

Biochem/physiol Actions

Ammonium sulfate is an inorganic salt, which is mostly used as a soil fertilizer. It is also used as a flame retardant since it elevates the combustion temperature of the material and lowers maximum weight loss rates. This leads to an upsurge in the production of residue or char.

Other Notes

The most commonly used salt for the fractional precipitation of proteins due to its solubility and effectiveness; Protein crystallization; Sepharose® chromatography of tRNAs using reverse salt gradients.

Legal Information

Sepharose is a registered trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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