Merck
All Photos(1)

Documents

126-2.5

SAFC

EHS Matrix Extract

Sign Into View Organizational & Contract Pricing

Select a Size

NACRES:
NA.75

form

liquid

Quality Level

shelf life

1 yr

shipped in

dry ice

storage temp.

−20°C

General description

EHS Matrix Extract is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. Its major components are laminin, collagen type IV, plaminogen activator and other growth factors which occur naturally in the EHS tumor.

Application

EHS Matrix Extract will gel rapidly at 22°C to 37°C. Thaw the EHS Matrix Extract at 4°C overnight in a refrigerator. Use pre-chilled pipettes, plates and tubes when preparing matrigel. Gelled EHS Matrix Extract may be liquefied by placing in refrigerator at 4°C overnight.

Preparation Note

Take the appropriate culture medium for your cells from the refrigerator. Decontaminate the bottle with 70% alcohol in a sterile hood.
*Keep EHS Matrix Extract on ice throughout the preparation. Use chilled pipette tips, pipettes, culture plates/flasks and tubes for preparing EHS Matrix Extract.
  • Thaw EHS Matrix Extract at 4°C overnight on ice.
  • Use cooled pipette tips, mix the EHS Matrix Extract on ice to homogeneity.
  • Prepare EHS Matrix Extract matrix by adding chilled culture medium to EHS Matrix Extract on ice in a 1:1 ratio.
  • Coat tissue culture surface evenly.
Keep the EHS Matrix Extract to surface ratio at 1ml/10cm2
For example:
2.5 ml for each T-25 flask (1.25 ml of culture medium to 1.25 ml of EHS Matrix Extract)
210 μl for each 24-well plate well (105 μL of culture medium to 105 μL of EHS Matrix Extract)
  • Incubate at 37°C for one hour.
  • Rinse EHS Matrix Extract with culture medium.
  • EHS Matrix Extract coated tissue culture surface is now ready for use.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Lital Livni et al.
Neuroscience letters, 694, 14-19 (2018-11-16)
Chemotherapy-induced peripheral neuropathy (CIPN) is a severe and debilitating adverse effect of cancer therapy that results from treatment with neurotoxic agents. Although chemotherapy treatment has been shown to inhibit neurite outgrowth from dorsal root ganglion (DRG) neurons in vitro, evidence
Zhen Yang et al.
Autophagy, 15(4), 668-685 (2018-11-06)
Emerging evidence has revealed that miRNAs could upregulate the expression levels of target genes. However, the molecular mechanism underlying upregulation of targets mediated by miRNAs remains unclear. In this study, we found a novel miRNA named MIR-G-1 by GRSF1-RNA immunoprecipitation
Richard Beatson et al.
Communications biology, 3(1), 644-644 (2020-11-06)
The tumour microenvironment plays a crucial role in the growth and progression of cancer, and the presence of tumour-associated macrophages (TAMs) is associated with poor prognosis. Recent studies have demonstrated that TAMs display transcriptomic, phenotypic, functional and geographical diversity. Here

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service