Merck
All Photos(1)

47036

Sigma-Aldrich

Saponin

for molecular biology, used as non-ionic surfactant

CAS Number:
EC Number:
MDL number:
NACRES:
NA.25

Quality Level

grade

for molecular biology

mol wt

1000-2000 g/mol

composition

Sapogenin, 8-25%

technique(s)

HPLC: suitable
protein quantification: suitable

CMC

0.001-0.01%

density

1.015-1.020 g/mL at 20 °C (5% in H2O)(lit.)

foreign activity

DNase, RNase, Protease, Phosphatase, none detected

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Application

Glycoside widely distributed in plants. Nonionic sufactant that forms oil-in-water emulsions and acts as protective colloid. Used for the direct determination of metals in milk by flame atomic-absorption spectrophotometry. Also used in permeabilization of cell membranes and separation of low molecular weight contaminants, and as an adjuvant in vaccine development.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Qingkun Liu et al.
Nature immunology, 20(8), 1023-1034 (2019-07-03)
Stroke is a multiphasic process in which initial cerebral ischemia is followed by secondary injury from immune responses to ischemic brain components. Here we demonstrate that peripheral CD11b+CD45+ myeloid cells magnify stroke injury via activation of triggering receptor expressed on
Sarah Costers et al.
Veterinary research, 40(5), 46-46 (2009-05-19)
The replication of porcine reproductive and respiratory syndrome virus (PRRSV) in lungs and lymphoid tissues of PRRSV-infected pigs is already strongly reduced before the appearance of neutralizing antibodies, indicating that other immune mechanisms are involved in eliminating PRRSV at those
Patcharaporn Tippayawat et al.
PLoS neglected tropical diseases, 3(4), e407-e407 (2009-04-09)
Infection with the Gram-negative bacterium Burkholderia pseudomallei is an important cause of community-acquired lethal sepsis in endemic regions in southeast Asia and northern Australia and is increasingly reported in other tropical areas. In animal models, production of interferon-gamma (IFN-gamma) is
Yixuan Fang et al.
Aging, 11(14), 4910-4922 (2019-07-23)
Autophagy has been well studied in regulating aging; however, the impact of autophagy in one organ on the aging of other organs has not been documented. In this study, we used a mouse model with deletion of an autophagy-essential gene
Shumin Tan et al.
PLoS pathogens, 5(5), e1000407-e1000407 (2009-05-05)
Helicobacter pylori (Hp) intimately interacts with the gastric epithelial surface and translocates the virulence factor CagA into host cells in a contact-dependent manner. To study how Hp benefits from interacting with the cell surface, we developed live-cell microscopy methods to

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