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5008

Sigma-Aldrich

VitroCol®

protein from human, suitable for cell culture

EC Number:

Quality Level

biological source

protein from human

sterility

sterile-filtered

form

lyophilized

packaging

pkg of 15 mg

manufacturer/tradename

Advanced BioMatrix

application(s)

cell culture | mammalian: suitable

impurities

<1 EU/mL Endotoxin level

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblasts to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.

VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. It contains high monomer content as measured by gel permeation chromatography.

VitroCol® is especially ideal for human cell culture systems when coating of surfaces and providing preparations of thin layers of culturing cells. It is not recommend for the formation of a solid gel. VitroCol® is supplied as a sterile, lyophilized powder.

Preparation Note

This product is supplied as a lyophilized powder with 15 mg of human collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

Legal Information

VitroCol is a registered trademark of Advanced BioMatrix, Inc.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Yasaman Barekatain et al.
Communications biology, 3(1), 328-328 (2020-06-27)
Magnetic resonance (MR) spectroscopy has potential to non-invasively detect metabolites of diagnostic significance for precision oncology. Yet, many metabolites have similar chemical shifts, yielding highly convoluted 1H spectra of intact biological material and limiting diagnostic utility. Here, we show that...

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