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5051

Sigma-Aldrich

Vitronectin

from human plasma, suitable for cell culture, solution

NACRES:
NA.75

Quality Level

biological source

human plasma

volume size

0.2 mL

sterility

sterile-filtered

assay

>95%

form

solution

mol wt

75 kDa

packaging

pkg of 0.1 mg

concentration

0.5 mg/mL

application(s)

cell culture | mammalian: suitable

solubility

water: miscible

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... VTN(7448)

Related Categories

General description

Vitronectin is a 75 kDa glycoprotein produced by hepatocytes and liberated into the blood. It has four domains and at least two structural motifs. Vitronectin gene is small with three kilobases in length and has eight exons. It is also present on fibers in the extracellular matrices of several tissues.
Vitronectin is a monomeric glycoprotein used to promote cell attachment, migration, proliferation and differentiation in a broad number of cell lines and types. This product has been purified from human plasma where it is found as a mixture of 75kDa and 65kDa polypeptides.

Vitronectin′s primary use in cell culture is related to cell adhesion. It also binds to heparin and collagen.

Vitronectin is ideal for coating of surfaces. The optimal concentration for cell attachment and culture may differ for various cell types. Vitronectin has been used at a final coating concentration as low as 50 ng/cm2 on plasticware. It is provided in user-friendly packaging for use and storage. Vitronectin is sterile filtered and is supplied as a ready to use solution after thawing and concentration adjustment.

Biochem/physiol Actions

Vitronectin controls proteolysis. It helps in in vitro cell adhesion.Vitronectin is involved in the modulation of perforin mediated killing by cytotoxic T-lymphocytes and natural killer (NK) cells. It controls the inflammatory reactions in blood.

Preparation Note

This product is supplied in a solution of 0.15 M NaCl and 0.005M HEPES at pH 7.4

Storage Class Code

12 - Non Combustible Liquids

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

The Molecular and Cellular Biology of Wound Repair (2013)
Extracellular Matrix (1996)
Zayda L Piedra-Quintero et al.
Frontiers in immunology, 9, 3118-3118 (2019-01-29)
Intestinal macrophages are highly mobile cells with extraordinary plasticity and actively contribute to cytokine-mediated epithelial cell damage. The mechanisms triggering macrophage polarization into a proinflammatory phenotype are unknown. Here, we report that during inflammation macrophages enhance its intercellular adhesion properties...
Si En Poh et al.
Frontiers in cellular and infection microbiology, 10, 148-148 (2020-04-25)
Malassezia is the most abundant eukaryotic microbial genus on human skin. Similar to many human-residing fungi, Malassezia has high metabolic potential and secretes a plethora of hydrolytic enzymes that can potentially modify and structure the external skin environment. Here we...
Augusto Martins Lima et al.
International journal of molecular sciences, 21(18) (2020-09-11)
Platelets play a crucial role in the immunological response and are involved in the pathological settings of vascular diseases, and their adhesion to the extracellular matrix is important to bring leukocytes close to the endothelial cells and to form and...

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