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51146

Sigma-Aldrich

Atto 550 Protein Labeling Kit

BioReagent, suitable for fluorescence

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NACRES:
NA.32

product line

BioReagent

manufacturer/tradename

ATTO-TEC GmbH

fluorescence

λex 544 nm; λem 576 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)

suitability

suitable for fluorescence

storage temp.

2-8°C

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This Item
6861651016MX555S100
Sigma-Aldrich

Sigma-Aldrich

51016

Atto 550 DPPE

fluorescence

λex 544 nm; λem 576 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)

fluorescence

λex 594 nm; λem 625 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)

fluorescence

-

fluorescence

λex 555 nm; λem 565 nm

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

product line

BioReagent

product line

BioReagent

product line

-

product line

-

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

-

General description

This kit contains sufficient amounts of reactive dye, buffers and protein purification sets for performing five labeling reactions (1 mg protein each) and for the subsequent purification of the labeled protein.

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550, which is similar to Cy3, may be useful in applications such as fluorescence resonance energy transfer (FRET) and as a tag for molecules such as secondary antibodies. Atto 550 Protein Labeling Kit contains Atto 550 succinimidyl ester which enables conjugation of Atto 550 to the primary amino groups of the proteins and other molecules.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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Atto Dyes and Tracy Dyes for Fluorescent Protein Labeling

We offer protein labeling kits based on two types of fluorescent dyes, the Atto dyes and the Tracy dyes. Both series of kits provide an easy and reliable way to fluorescently label purified proteins, enzymes, and antibodies.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service