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5170

Sigma-Aldrich

ECM Select®

Array Kit Ultra - 36

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Synonym(s):
Extracellular matrix screening array
NACRES:
NA.75

biological source

bovine

shipped in

wet ice

storage temp.

−20°C

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GBL204830ABK0001HPA027241
ECM Select® Array Kit Ultra - 36

Sigma-Aldrich

5170

ECM Select®

Sigma-Aldrich

Sigma-Aldrich

ABK0001

AvaBridge ELISA Kit

biological source

bovine

biological source

-

biological source

-

biological source

rabbit

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

wet ice

General description

ECM Select® Array Kit Ultra-36 is an extracellular matrix screening array. This product has 36 extracellular matrix (ECM) conditions that are deposited onto the hydrogel surface as printed array spots. Each ECM condition is printed with 9 replicate spots where each spot has diameter of 400 um. The ECMs are localized within each spot without cross diffusion from neighboring spots, therefore each spot represents an independent ‘well′ or experiment.

The ECM Select® Array Kit Ultra-36 contains a microscope glass slide printed with the ECM conditions contained within a protective slide holder and a pre-sterilized tray. The components are sterilized and ready-to-use.

Cells of interest are seeded on to the ECM Select® Array slide and allowed to be cultured in the incubator for the desired amount of time. Cell morphology, attachment and growth can be visualized using a bright field microscope. Specific cellular behaviors can be monitored by staining the cells on the slide using specific fluorescence based markers. Fluorescence signal can be detected using a fluorescence microscope imaging system.

The ECM Select® Array Kit Ultra-36 is simple to use in comparison to performing the same experiment in multi-well tissue culture plates. Cell seeding, fixing, washing and process steps are done in one single solution exchange, eliminating multi-well solution handlings. From start to finish, the optimal ECM for culturing cells can be determined in less than 24 hours for most cell types.

Legal Information

ECM Select is a registered trademark of Advanced BioMatrix, Inc.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

444.2 °F - closed cup

Flash Point(C)

229.00 °C - closed cup


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Tongfei Liu et al.
The Journal of experimental medicine, 217(12) (2020-09-18)
Variations in many genes linked to sporadic Alzheimer's disease (AD) show abundant expression in microglia, but relationships among these genes remain largely elusive. Here, we establish isogenic human ESC-derived microglia-like cell lines (hMGLs) harboring AD variants in CD33, INPP5D, SORL1

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