Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.
The BioChemika quality is for the usual biochemical applications. The products designated as BioChemika Ultra grade are suitable for different applications like purification, precipitation, crystallization and other applications which require tight control of elemental content. Trace elemental analyses have been performed for all qualities. The molecular biology qualities are also tested for absence of nucleases and the luminescence quality spectroscopic tests are performed.
Trizma has been used:
- In the dissection medium and culture medium of Hippocampal organotypic tissue cultures
- As a component of lysis buffer before immunoprecipitation and immunoblotting
- As a component of harvest buffer, lysis buffer, ChIP dilution buffer, and wash buffer in chromatin immunoprecipitation method for studying protein-DNA binding
- As a component of mixed buffer formulations
- As a component of NuPAGE loading buffer for SDS-PAGE and as a component of transfer buffer for Western blotting
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma base is used as a general reagent for the preparation of all types of Tris buffers.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Easily compare specifications for Trizma products with the Trizma specification table.
Running buffer component in non-denaturing agarose gel electrophoresis; In non-denaturing and urea-denaturing polyacrylamide gel electrophoresis; DNA digest analysis with capillary electrophoresis.
Trizma is a registered trademark of Sigma-Aldrich Co. LLC