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A2095
Monoclonal Anti-HA−Agarose antibody produced in mouse
clone HA-7, purified immunoglobulin, PBS suspension
Synonym(s):
Monoclonal Anti-HA, Anti-HA, Anti-Influenza Hemagglutinin
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About This Item
Conjugate:
agarose conjugate
Clone:
HA-7, monoclonal
Application:
Citations:
621
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Product Name
Monoclonal Anti-HA−Agarose antibody produced in mouse, clone HA-7, purified immunoglobulin, PBS suspension
biological source
mouse
conjugate
agarose conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
HA-7, monoclonal
form
PBS suspension
isotype
IgG1
capacity
20-50 nmol/mL elution capacity (HA-tagged fusion protein)
30-50 nmol/mL binding capacity (HA-tagged fusion protein)
shipped in
wet ice
storage temp.
2-8°C
Quality Level
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This Item | H3663 | A5477 | A1970 |
|---|---|---|---|
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified from hybridoma cell culture | antibody form purified immunoglobulin |
| clone HA-7, monoclonal | clone HA-7, monoclonal | clone HA-7, monoclonal | clone P5D4, monoclonal |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| conjugate agarose conjugate | conjugate unconjugated | conjugate alkaline phosphatase conjugate | conjugate agarose conjugate |
| form PBS suspension | form - | form buffered aqueous glycerol solution | form PBS suspension |
| shipped in wet ice | shipped in dry ice | shipped in wet ice | shipped in wet ice |
Application
Suitable for use in immunoprecipitation assays and for immunoaffinity purification of HA-tagged fusion proteins.
Biochem/physiol Actions
The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells.
General description
The HA tag is a 9-amino acid tag, with the sequence YPYDVPDYA (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C), derived from the hemagglutinin (HA) protein at amino acid residues 98-106 in the HA sequence. The HA tag contains a high proportion of charged amino acid residues, which makes the HA tag likely to form a strong antibody recognition site. Monoclonal Anti-HA Agarose Conjugate recognizes native as well as denatured/reduced forms of HA-tagged proteins. This product is reactive with N-or C-terminal HA-tagged fusion proteins that are expressed in E. coli or in mammalian cells.
Immunogen
synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH
Physical form
Suspension in 0.01 M phosphate buffered saline, containing 15 mM sodium azide
Preparation Note
Prepared using cyanogen bromide-activated agarose. Specific antibody concentration is 2.0 - 2.4 mg/mL settled resin.
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Pricing
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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Lin-Chun Chang et al.
Nucleic acids research, 40(3), 1331-1344 (2011-10-15)
The yeast RNA helicase Dhh1p has been shown to associate with components of mRNA decay and is involved in mRNA decapping and degradation. An RNA-binding protein, Rbp1p, is known to bind to the 3'-UTR of porin (POR1) mRNA, and induces
Frontiers in neurology and neuroscience research null
Ying Li et al.
Cancer research, 70(20), 8159-8168 (2010-10-05)
As a tumor marker for colorectal cancers, carcinoembryonic antigen (CEA) enhances the metastatic potential of cancer cells. CEA functions as an intercellular adhesion molecule and is upregulated in a wide variety of human cancers. However, the molecular mechanisms by which
Yinghong Ma et al.
Journal of virology, 85(1), 86-97 (2010-10-22)
Many aspects of the assembly of hepatitis C virus (HCV) remain incompletely understood. To characterize the role of NS2 in the production of infectious virus, we determined NS2 interaction partners among other HCV proteins during productive infection. Pulldown assays showed
Michael W Baker et al.
Developmental biology, 344(1), 346-357 (2010-06-15)
LAR-like receptor protein tyrosine phosphatases (RPTPs), which are reported to be highly expressed in the nervous systems of most bilaterian animals, have been implicated in the regulation of critical developmental processes, such as neuronal pathfinding, cell adhesion and synaptogenesis. Here
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What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?
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Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.
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