Merck
All Photos(5)

Documents

A2153

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, ≥96% (agarose gel electrophoresis)

Sign Into View Organizational & Contract Pricing

Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

biological source

bovine

Quality Level

Assay

≥96% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

~66 kDa

purified by

cold ethanol fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

ELISA: suitable
blood typing: suitable
microbiological culture: suitable

impurities

≤6.0% Loss on Drying

loss

≤6%

pH

6.5-7.5 (1% in 0.15 M sodium chloride)
7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

foreign activity

BT Virus, none detected
VSV Virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

Looking for similar products? Visit Product Comparison Guide

General description

Bovine Serum Albumin (BSA) is a globular, non-glycosylated α-helical plasma protein produced in the bovine liver. It consists of three domains (I, II, and III), each with two sub-domains (A and B), arranged in a heart-shaped structure.

Application

Bovine serum albumin has been used-

  • along with BTS (Beltsville thawing solution), for the extension of sperms during sperm motion analysis
  • as a component of modified Krebs-Ringer buffer (dmKRBT)
  • in gel shift analysis
  • as a blocking agent in immunocytochemistry, immunohistochemistry,(4) immunofluorescence protocol and sandwich enzyme-linked immunosorbent assay (ELISA)
  • as a component of phosphate buffer saline (PBS) solution to resuspend cells for analysis during flow cytometry
  • as a protein standard to quantify proteins in tissue samples via Bradford’s method
  • to measure the enzyme activity of 17β HSD enzyme

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service