Merck
All Photos(1)

A2220

Millipore

ANTI-FLAG® M2 Affinity Gel

purified immunoglobulin, buffered aqueous glycerol solution

Synonym(s):
Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG M2 antibody produced in mouse, ANTI-FLAG M2 Affinity Agarose Gel
NACRES:
NA.32

Quality Level

conjugate

agarose conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous glycerol solution

analyte chemical class(es)

proteins (FLAG® tag, 3x FLAG®, DYKDDDDK tag)

technique(s)

affinity chromatography: suitable (FLAG® peptide, Glycine, pH3.5, 3x FLAG® peptide)
immunoprecipitation (IP): suitable

matrix

(4% agarose bead; 45-165μm bead size)

isotype

IgG1

capacity

>0.6 mg/mL, resin binding capacity (FLAG-BAP)

shipped in

wet ice

storage temp.

−20°C

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General description

Anti-FLAG M2 Affinity gel is a mouse monoclonal antibody that is covalently attached to agarose. The antibody binds FLAG at the N-terminal, Met-N-terminal, C-terminal and internal locations of fusion proteins. Binding is calcium-independent.

Elution - FLAG® peptide, Glycine, pH 3.5, 3x FLAG® peptide

Immunogen

DYKDDDDK

Application

Anti-FLAG® M2 affinity gel has been used for western blotting, immunoprecipitation and for the purification of FLAG fusion proteins.

Browse additional application references in our FLAG® Literature portal.

Physical form

Suspension in buffered saline containing azide as preservative and 50% glycerol

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Articles

ANTI-FLAG® M2 Magnetic Beads

The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.

Protocols

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

Related Content

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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