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A3294

Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, protease free, pH 7, ≥98%

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Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

biological source

bovine

Quality Level

Assay

≥98%

form

lyophilized powder

mol wt

~66 kDa

purified by

heat shock fractionation

packaging

poly bottle of

origin

USA origin

technique(s)

ELISA: suitable
blood typing: suitable
microbiological culture: suitable

impurities

protease, none detected

loss

≤5%

pH

7

solubility

water: soluble (40 mg/ml)

UniProt accession no.

foreign activity

BT Virus, none detected
VSV Virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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This Item
A3059A7030A8022
Bovine Serum Albumin heat shock fraction, protease free, pH 7, ≥98%

A3294

Bovine Serum Albumin

Bovine Serum Albumin heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%

A3059

Bovine Serum Albumin

Bovine Serum Albumin heat shock fraction, protease free, fatty acid free, essentially globulin free, pH 7, ≥98%

A7030

Bovine Serum Albumin

Bovine Serum Albumin heat shock fraction, pH 5.2, ≥96%

A8022

Bovine Serum Albumin

assay

≥98%

assay

≥98%

assay

≥98%

assay

≥96%

biological source

bovine

biological source

bovine

biological source

bovine

biological source

bovine

origin

USA origin

origin

USA origin

origin

USA origin

origin

USA origin

Quality Level

300

Quality Level

300

Quality Level

300

Quality Level

300

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

General description

Albumin is abundantly present in human plasma. Increased levels of albumin result in dehydration. Albumin turnover is usually found in children with iron deficiency anemia. It is a predictive indicator for liver disease.

Application

Bovine Serum Albumin has been used:
  • to block non-specific binding of the antibodies
  • as a component of activating medium for inducing sperm motility
  • to determine the protein concentration of the bacteriocin by the bicinchoninic acid (BCA) method

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Heat shock fractionated
  • Protease-free

Preparation Note

Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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