A4403

L-Ascorbic acid

powder, suitable for cell culture, γ-irradiated

• Synonym(s): L-Threoascorbic acid, Antiscorbutic factor, Vitamin C
• Empirical Formula (Hill Notation): C₆H₈O₆
• CAS Number: 50-81-7
• Molecular Weight: 176.12
• Beilstein: 84272
• EC Number: 200-066-2
• MDL number: MFCD00064328
• PubChem Substance ID: 24890857
• NACRES: NA.75

Properties

• biological source: synthetic (organic)
• Quality Level: 200
• sterility: γ-irradiated
• form: powder
• mol wt: M_w 176.12 g/mol
• technique(s): cell culture | insect: suitable; cell culture | mammalian: suitable; cell culture | plant: suitable
• color: white to off-white
• pH: 1.0-2.5 (25 °C, 176 g/L in water)
• mp: 190-194 °C (dec.)
• solubility: H₂O: 10 mg/mL
• SMILES string: OC([C@]([C@@H](O)CO)([H])O1)=C(O)C1=O
• InChI: 1S/C6H8O6/c7-1-2(8)5-3(9)4(10)6(11)12-5/h2,5,7-10H,1H2/t2-,5+/m0/s1
• InChI key: CIWBSHSKHKDKBQ-JLAZNSOCSA-N
• Gene Information: human ... SLC23A2(9962)

Description

General description

L-ascorbic acid is an unsaturated γ-lactone ring containing an enediol configuration (–COH=COH) attached with a carbonyl group.

Application

For use in cell culture applications as an antioxidant and to regenerate tocopherol.
L-ascorbic acid has been used:

• in an osteogenic supplement for the growth of cells
• as a component in the differentiation media
• in WS-MSCs (Werner syndrome-mesenchymal stem cells) to study rescuing properties of ascorbic acid on aging defects

Packaging

100 mg

Biochem/physiol Actions

L-ascorbic acid mainly exhibits antioxidant properties. It protects plants from oxidative stress and mammals from diseases associated with oxidative stress. L-ascorbic acid mainly protects from hydroxyl radicals, superoxide and singlet oxygen. In addition, it also reduces the membrane-linked antioxidant α-tocopherol (oxidised form). L-Ascorbic acid enhances endothelium-dependent vasodilation in various disorders, including diabetes, coronary artery disease, hypertension and chronic heart failure.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves