A5503

Sigma-Aldrich

Albumin from chicken egg white

lyophilized powder, ≥98% (agarose gel electrophoresis)

Synonym(s):
Albumin from hen egg white, Ovalbumin
CAS Number:
EC Number:
MDL number:
eCl@ss:
42010201
NACRES:
NA.25

Quality Level

biological source

chicken egg white

type

Grade V

assay

≥98% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

44287 Da by calculation

purified by

crystallization

application(s)

bioconjugation: suitable

solubility

H2O: soluble 40 mg/mL

UniProt accession no.

storage temp.

2-8°C

Gene Information

chicken ... OVAL(396058)

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General description

Chicken egg albumin, or ovalbumin, is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated glycoprotein. From the amino acid sequence, the peptide portion of ovalbumin consists of 385 residues and has a molecular weight of 42.7 kDa.

Application

Ovalbumin is useful as a marker (∼45 kDa) for protein electrophoresis. Ovalbumin has been used to produce an animal model of asthma. Ovalbumin was used as a medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophils.
Studies to test the effects of localization on DNA immunizations use ovalbumin in antibody assays.
Ovalbumin has been used as an induction agent in model studies of atopic dermatitis.
A phosphorylated glycoprotein which is useful as a marker (~45 kDa) for protein electrophoresis. Has been used to produce an animal model of asthma.

Packaging

1, 5, 10, 25, 50 g in poly bottle

Biochem/physiol Actions

Differential scanning calorimetry indicates that chicken egg albumin denatures at 84 °C. Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen.

Physical form

Essentially salt free

pictograms

Health hazard

signalword

Danger

hcodes

hazcat

Resp. Sens. 1A

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 2

Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

  1. Is Product No. A5503, Albumin from chicken egg white, endotoxin-tested?

    A5503 is not endotoxin-tested. None of the ovalbumins are endotoxin-tested.

  2. What is the solubility of Product No. A5503, Albumin from chicken egg white, in aqueous buffers (e.g. Tris, PBS)?

    We have only tested solubility in water. Salts in buffers may make solubility more difficult.

  3. How is Product No. A5503, Albumin from chicken egg white, analyzed for purity?  

    A5503 is analyzed by agarose gel electrophoresis. We have not done SDS-PAGE.

  4. Product No. A5503, Albumin from chicken egg white, is to be stored at 2 - 8°C. Does it ship on ice?

    A5503 does not require shipping on ice.

  5. Is Product No. A5503, Albumin from chicken egg white, suitable for cell culture or injection?

    We do not test any of the ovalbumins in cell culture or in vivo applications. Customers would need to perform suitability testing in their own experiments.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

J S Boyle et al.
International immunology, 9(12), 1897-1906 (1998-02-18)
We examined the role of the cellular localization of antigen on the immune response after DNA immunization of mice with three forms of ovalbumin (OVA). DNA encoding OVA which was secreted (sOVA) generated 10- to 100-fold higher IgG responses with...
Ken Morishima et al.
Communications biology, 3(1), 294-294 (2020-06-10)
Currently, a sample for small-angle scattering (SAS) is usually highly purified and looks monodispersed: The Guinier plot of its SAS intensity shows a fine straight line. However, it could include the slight aggregates which make the experimental SAS profile different...
Christianne Bandeira-Melo et al.
Journal of immunological methods, 276(1-2), 227-237 (2003-05-10)
Eosinophils contain several preformed cytokines within their specific granules. Therefore, without requiring them in de novo synthesis of cytokines, eosinophils can release quantities of granule-derived cytokines by highly regulated mechanisms. However, eosinophil "degranulation" is poorly understood, in part, because available...
A D Nisbet et al.
European journal of biochemistry, 115(2), 335-345 (1981-04-01)
The complete amino acid sequence of hen ovalbumin, comprising 385 residues, has been determined. The sequence was deduced from the 17 cyanogen bromide fragments and from peptides derived by digestion with a number of proteolytic enzymes. The molecular weight of...
Ana Raquel Maceiras et al.
Nature communications, 8, 15067-15067 (2017-04-22)
Immunization leads to the formation of germinal centres (GCs) that contain both T follicular helper (Tfh) and T follicular regulatory (Tfr) cells. Whether T-cell receptor (TCR) specificity defines the differential functions of Tfh and Tfr cells is unclear. Here we...
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