buffered aqueous glycerol solution
indirect ELISA: 1:20,000
human ... GPX1(2876)
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Enter Lot Number to search for Certificate of Analysis (COA).
Enter Lot Number to search for Certificate of Origin (COO).
The product cannot be used on paraffin embedded tissue sections, but it does work on fixed culture cells (as indicated on the Product Information Sheet) and also may work on frozen sections.
An antibody titer from 1:100 to 1:1000 is recommended for immunocytochemistry and Western blot. Minimum antibody titer recommended for ELISA is 1:20,000. Adjust antibody titer to maximize detection sensitivity and to minimize background.
This product ships on wet ice and it is recommended to store the undiluted antibody at 0 to -20°C. Once diluted, repeated freezing and thawing is not recommended.
There is a troubleshooting guide provided at the end of the product information sheet. Some suggestions include the following: adjusting antibody titer, increase number of washes, increase stringency of washes by addition of 0.05% TWEEN 20 or 0.1% TRITON X-100 to the wash buffers, and/or increase the incubation and wash temperatures.
Product No. A8592, ANTI-FLAG M2-peroxidase is supplied as a solution in 10 mM sodium phosphate, pH 7.4, containing 150 mM NaCl and 50% glycerol, plus stabilizer and preservative. The conjugate protein concentration is approximately 1 mg/ml.
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Ask a Scientist here.
Structural modifications of proteins are essential to living cells. When aberrantly regulated they are often the basis of disease. Glycans are responsible for much of the structural variation in biologic systems, and their representation on cell surfaces is commonly called the “glycome.”