A8806

Sigma-Aldrich

Bovine Serum Albumin

fatty acid free, low endotoxin, lyophilized powder, BioReagent, suitable for cell culture, ≥96% (agarose gel electrophoresis)

Synonym(s):
Albumin bovine serum, Bovine albumin, BSA
CAS Number:
EC Number:
MDL number:
NACRES:
NA.27

Quality Level

biological source

bovine

product line

BioReagent

assay

≥96% (agarose gel electrophoresis)

form

lyophilized powder

mol wt

~66 kDa

purified by

cold ethanol fractionation

packaging

poly bottle of

origin

USA origin

free fatty acid content

≤0.02%

application(s)

cell culture | mammalian: suitable

impurities

≤0.02% fatty acid (GC)
≤0.1 ng/mg endotoxin

loss

≤5%

pH

4.8-7.5

solubility

water: soluble (40 mg/ml)

UniProt accession no.

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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General description

Bovine serum albumin (BSA) is a water-soluble serum protein, 583 amino acids in length, with a calculated molecular weight of 66,430 Daltons. Six a-helices form its three homologous domains. Depending on pH, bovine serum albumin undergoes reversible conformational isomerization. BSA native structure becomes reactive and flexible on heating.

BSA is a carrier protein for many biomolecules, such as fatty acids, amino acids, and steroids. For its carrier properties, BSA is also widely used in cell culture. BSA also serves antioxidant functions when used in cell culture.

Application

Bovine Serum Albumin has been used in the solution prepared for digestion of tissues. It has been used as a blocking agent in cell spreading assay.

Because BSA is a well-known carrier of fatty acids (FA), control over the particular fatty acids used to culture cell lines is important, for cell culture lines which are sensitive to particular fatty acids. Fatty acid-free BSA is a highly useful reagent for such cell culture experiments, to allow researchers to use such BSA as a carrier for their specific fatty acids specific to their cell culture system. This mitigates potential risks to the cells from FA′s that might be present in non-FA-free BSA.

This BSA product is specifically tested for the following properties:
  • Fatty acid free / very low FA content
  • Low endotoxin level
  • Suitability to use in cell culture

Packaging

1, 5 g in poly bottle

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Tested for use in cell culture
  • Fatty acid-free
  • Low endotoxin
  • Cold ethanol fractionated

Preparation Note

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Hajime Abe et al.
Nature communications, 10(1), 2824-2824 (2019-06-30)
The fibrogenic response in tissue-resident fibroblasts is determined by the balance between activation and repression signals from the tissue microenvironment. While the molecular pathways by which transforming growth factor-1 (TGF-β1) activates pro-fibrogenic mechanisms have been extensively studied and are recognized...
Simon A Johnston et al.
BMC cell biology, 9, 65-65 (2008-12-11)
Cells use filopodia to explore their environment and to form new adhesion contacts for motility and spreading. The Arp2/3 complex has been implicated in lamellipodial actin assembly as a major nucleator of new actin filaments in branched networks. The interplay...
Extracellular stimulation of mammalian neurons through repetitive activation of Na+ channels by weak capacitive currents on a silicon chip.
Schoen I and Fromherz P
Journal of Neurophysiology, 100, 346-346 (2008)
Corrigendum: Formation and reshuffling of disulfide bonds in bovine serum albumin demonstrated using tandem mass spectrometry with collision-induced and electron-transfer dissociation.
Rombouts I et al.
Scientific Reports, 5 (2015)
Judith A Stolwijk et al.
The Journal of biological chemistry, 291(44), 22894-22912 (2016-10-30)
Endothelial barrier function is tightly regulated by plasma membrane receptors and is crucial for tissue fluid homeostasis; its dysfunction causes disease, including sepsis and inflammation. The ubiquitous activation of Ca2+ signaling upon phospholipase C-coupled receptor ligation leads quite naturally to...
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