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B8438

Sigma-Aldrich

Alcian Blue solution

1% in 3% acetic acid, pH 2.5

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Synonym(s):
Alcian Blue 8XG solution
NACRES:
NA.47

form

liquid

Quality Level

concentration

1% in 3% acetic acid, pH 2.5

pH range

2.4-2.6

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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This Item
32857A3157B8563
Alcian Blue solution 1% in 3% acetic acid, pH 2.5

Sigma-Aldrich

B8438

Alcian Blue solution

Wright Stain solution according to Wright

Sigma-Aldrich

32857

Wright Stain solution

Alcian Blue 8GX certified by the Biological Stain Commission

Sigma-Aldrich

A3157

Alcian Blue 8GX

Sigma-Aldrich

Sigma-Aldrich

B8563

Aniline Blue solution

concentration

1% in 3% acetic acid, pH 2.5

concentration

-

concentration

-

concentration

2.5% in 2% acetic acid

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

-

pH range

2.4-2.6

pH range

-

pH range

-

pH range

-

Application

Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.

Biochem/physiol Actions

Alcian Blue is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby aiding in the quantitative determination of glycosaminoglycans. It is also used for the staining of bone and cartilage.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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A procedure for differential staining of cartilage and bone in whole formalin-fixed vertebrates.
Wassersug R J.
Stain Technology, 51(2), 131-134 (1976)
SPECIFIC STAINING OF SULPHATE GROUPS WITH ALCIAN BLUE AT LOW PH.
R LEV et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 12, 309-309 (1964-04-01)
The quantitative determination of glycosaminoglycans in urine with Alcian Blue 8GX.
Whiteman P.
The Biochemical Journal, 131(2), 351-357 (1973)
W Lin et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45(8), 1157-1163 (1997-08-01)
The apparent intensity of hyaluronan (HA) staining in tissue sections can vary as a function of fixation techniques. We examined the histochemical distribution of HA in normal human skin using an HA-specific binding peptide derived from bovine nasal cartilage. The
Differential staining of acid glycosaminoglycans (mucopolysaccharides) by alcian blue in salt solutions.
J E Scott et al.
Histochemie. Histochemistry. Histochimie, 5(3), 221-233 (1965-10-01)

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