Merck
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C0897

Sigma-Aldrich

Citrate Lyase from Klebsiella pneumoniae

lyophilized powder, ≥0.20 unit/mg solid

Synonym(s):
Citrate oxaloacetate lyase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

form

lyophilized powder

Quality Level

specific activity

≥0.20 unit/mg solid

mol wt

>5 kDa

storage temp.

2-8°C

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Application

Citrate Lyase from Klebsiella pneumoniae has been used in the digestion of low molecular weight human milk fraction (>5 kDa referred to as 5kF).

Packaging

100 units in poly bottle

Biochem/physiol Actions

Citrate lyase is found in several microorganisms and catalyzes the first step of citrate degradation, forming acetate and oxaloacetate. The enzyme contains 3 polypeptide subunits, α-subunit (a transferase), β-subunit (acyl lyase) and γ-subunit (acyl-carrier protein).

Unit Definition

One unit will convert 1.0 μmole of citrate to oxalacetate per min at pH 7.6 at 25 °C.

Physical form

Lyophilized powder containing bovine serum albumin, sucrose, and MgSO4

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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