C9301

Collagen from chicken sternal cartilage

Type II (Miller), powder, BioReagent, suitable for cell culture

• CAS Number: 9007-34-5
• EC Number: 232-697-4
• MDL number: MFCD00130825
• NACRES: NA.75

Properties

• Quality Level: 300
• biological source: chicken (Sternal cartilage)
• sterility: non-sterile
• type: Type II (Miller)
• product line: BioReagent
• assay: 60-80% (biuret)
• form: powder
• packaging: glass bottle of 100 mg; poly bottle of 25 mg; glass bottle of 5 mg
• technique(s): cell culture | mammalian: suitable
• surface coverage: 6‑10 μg/cm²
• solubility: acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)
• UniProt accession no.: P02460
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: chicken ... COL2A1(395069)

Description

General description

Collagen is made of three polypeptide chains. Type 2 collagen is ubiquitously present in the hyaline cartilage. It is a homotrimer made of three α1 chains. Type 2 collagen is also predominant in nucleus pulposus of the vertebral disc and vitreous body of the eye. The proteoglycan obtained from the chicken sternal cartilage is similar to type nine collagen structure.

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Application

Collagen from chicken sternal cartilage has been used in enzyme–linked immunosorbent assay (ELISA) , chondrocyte-mediated tissue production in vitro, induction, treatment and assessment of collagenα induced arthritis (CIA) and cell proliferation assay.

Packaging

25 mg in poly bottle

5 mg in glass bottle

100 mg in glass bottle

Other Notes

Major component of articular cartilage.

Preparation Note

Prepared by a modification of the method of Trentham, D.E., et al.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves