1-7HB2

10081201, from human breast

Properties

• biological source: human breast
• growth mode: Adherent
• karyotype: Not specified
• morphology: Cuboidal
• products: Formation of branching structures in the presence of fibroblast cultures or fibroblast-conditioned medium. Formation of spheroids in collagen type I gels.
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice

Description

Cell Line Origin

Breast cancer, morphology, adhesion properties

Cell Line Description

1-7HB2 is a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7 identified by screening for branching morphology. The MTSV1-7 is a clonal non-tumourigenic cell line developed by immortalizing luminal epithelial cells cultured from milk using the SV40 T antigen. (See also MTSV1-7 CE1 Sigma catalog number 10081202.). Used for in vitro study of branching morphogenesis of human mammary epithelial cells and the role of integrins in mammary morphogenesis.

DNA Profile

STR-PCR Data:
Amelogenin: X
CSF1PO: 12,13
D13S317: 12
D16S539: 8,13
D5S818: 10,13
D7S820: 8,10
THO1: 7,9
TPOX: 11
vWA: 14,17

Culture Medium

DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + Hydrocortisone (5 μg/ml) + Bovine Insulin (10 μg/ml).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Other Notes

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Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.