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FLAG® M Purification Kit

For Mammalian expression systems.

Anti-ddddk, Anti-dykddddk

Quality Level

shipped in

wet ice

storage temp.



1 kit
Sufficient for 3-5 uses of 1-ml affinity purification column.


The FLAG Purification Kit utilizes CelLytic Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG® M2 affinity gel for affinity purification of active FLAG fusion proteins.

Browse additional application references in our FLAG® Literature portal.

Features and Benefits

  • Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins.
  • ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations.
  • Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide).

Other Notes

Kit contains CelLytic™ M for cell lysis and protein extraction from Mammalian cells.

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.

  • C2978CelLytic M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.SDS

  • SAE0194Purified 3xFLAG® peptide, ≥95% (HPLC), lyophilized powderSDS

  • A2220ANTI-FLAG® M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solutionSDS

  • C2103Chromatography columns, general-purpose, volume 10 mL, Overall H 5 in.SDS

Storage Class Code

10 - Combustible liquids



Certificate of Analysis

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Certificate of Origin

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Product Information Sheet

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